在活体炎症模型中进行硫化成像的新型光探针
Xin-Ai Shi1, Yu Jiang1, Pei Tang1
1Institute of Pharmaceutical Biotechnology, School of Biology and Food Engineering, Suzhou University, Suzhou 234000, PR China. yeyaxi666@163.com.
Analytical methods : advancing methods and applications
|February 26, 2026
概括
研究人员开发了DHM-H2S,这是一个新的光探针,用于实时检测硫化 (H2S). 这种工具可以在炎症细胞和动物模型中对H2S进行敏感的成像,从而推进H2S生物学研究.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 硫化 (H2S) 在生物过程中至关重要,需要有效的检测方法.
- 现有的工具缺乏对H2S在复杂生物系统中的选择性或实时能力.
研究的目的:
- 开发一种新的光探测器DHM-H2S,用于选择性和敏感的实时H2S检测.
- 为了验证探针在炎症细胞,C. elegans和小鼠模型中成像H2S的有效性.
主要方法:
- 基于H2S触发的裂变的DHM-H2S探头的设计和合成.
- 光光谱和显微镜用于探针表征和成像.
- 在体外测定与巨细胞和体内研究在C. elegans和小鼠.
主要成果:
- DHM-H2S显示出对H2S的高选择性,检测极限低 (2.38μM).
- 探测器成功地可视化了LPS刺激的巨细胞中的内源H2S波动.
- 实时H2S监测在活生生的C. elegans和小鼠模型中实现.
结论:
- DHM-H2S是一个强大的工具,用于在各种生物系统中实时H2S成像.
- 探测器的特性支持其用于研究与H2S相关的炎症和衰老过程.
- 这个平台促进了H2S生物学研究以及在疾病和药物开发中的潜在应用.
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