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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

19.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.3K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

808
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
808
Homologous Recombination02:31

Homologous Recombination

64.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.3K

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相关实验视频

Updated: Feb 27, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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基于CRISPR/Cas系统的生物传感器

Xingjie Hu1, Jing Su2, Shiping Song3

  • 1State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Biosensors
|February 26, 2026
PubMed
概括
此摘要是机器生成的。

聚类定期间隔的短平行列重复 (CRISPR) 和与CRISPR相关的 (Cas) 蛋白质已经改变了生物研究. 这些系统最初在细菌中发现,为基因操纵和科学发现提供了强大的工具.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 微生物学 微生物学

背景情况:

  • 聚类定期间隔的短平行列重复 (CRISPR) 和与CRISPR相关的 (Cas) 蛋白质是细菌的防御机制.
  • 这些系统准和中和入侵的核酸.

研究的目的:

  • 审查CRISPR-Cas系统对生物研究的革命性影响.
  • 要突出其应用超出其自然功能.

主要方法:

  • 对CRISPR-Cas研究的文献评论.
  • 分析CRISPR-Cas在各种生物领域的应用.

主要成果:

  • 克里斯普尔-卡斯技术已成为现代实验室中不可或缺的工具.
  • 应用范围包括基因编辑,诊断和治疗开发.

结论:

  • 克里斯普尔-卡斯系统从根本上改变了生物研究方法.
  • 它们的多功能性保证了生命科学领域的持续创新.