快速检测Fumonisin B1使用光Aptasensor与等离子体修饰的氧化石墨烯氧化物作为一个消火器
Yi Jiao1,2, Xiaoqing Yang3, Junping Hao1,2
1Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Biosensors
|February 26, 2026
概括
一个新的生物传感器能够有效地检测到一种危险的真菌毒素 - - 烟素B1 (FB1). 这种基于aptamer的传感器提供了一种简单,灵敏的方法来检测食品样本中的FB1.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 富蒙尼辛B1 (FB1) 是一种来自Fusarium物种的有毒二次代谢物,被归类为2B组致癌物.
- FB1对人类和动物健康构成重大风险,需要可靠的检测方法.
- 目前的检测方法可能很复杂,需要更简单,更有效的分析工具.
研究的目的:
- 开发一种简单可靠的生物传感器,用于检测和分析Fumonisin B1 (FB1).
- 为了利用血改性石墨烯氧化物 (mGO) 和核酸吸收剂进行敏感的FB1量化.
- 为了验证生物传感器在现实世界食品样本矩阵中的性能.
主要方法:
- 使用血改性石墨烯氧化物 (mGO) 作为光灭剂和使用FAM标记的光体 (FAM-APT) 构建了一个生物传感器.
- 使用光共振能量转移 (FRET),其中FB1与FAM-APT结合导致从mGO解离,从而导致光恢复.
- 通过测量光强度的变化来实现定量检测,以响应不同的FB1度.
主要成果:
- 生物传感器表现出极好的线性,从10到5×10^6 ng/L,其低检测极限 (LOD) 为0.16 μg/L.
- 传感器显示FB1对其他六种常见的真菌毒素具有很高的特异性.
- 实践样本分析显示,玉米 (95.8104.7%) 和大米 (89.394.5%) 样本中FB1的恢复率很高.
结论:
- 开发的基于aptamer的生物传感器为FB1.1提供了一种简单,高度敏感和广泛的检测方法.
- 这项技术为推进真菌毒素研究和食品安全分析提供了至关重要的技术支持.
- 生物传感器在真实样本中的性能凸显了其在真菌毒素监测中的实际应用潜力.
相关概念视频
01:19Gas Chromatography: Types of Detectors-II
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
01:25Atomic Absorption Spectroscopy: Atomization Methods
Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
01:30Atomic Emission Spectroscopy: Interference
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
01:29Atomic Fluorescence Spectroscopy
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
01:02Flame Photometry: Overview
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...