在转移性肝细胞母细胞瘤中,lncRNAMEG3的升调

相关实验视频

Updated: Feb 27, 2026

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.9K

Morgan L Brown¹, Maryam G Shaikh¹, Nazia Nazam¹

¹Division of Pediatric Surgery, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35233, USA.

Cells

|February 26, 2026

概括

母体表达的基因3 (MEG3) 长非编码RNA在转移性肝细胞瘤中被上调. MEG3促进癌细胞生长,迁移和入侵,这表明它可能是侵袭性儿科肝癌的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

肝母细胞瘤是最常见的儿科肝癌.
对于转移性肝母细胞瘤患者来说,结果很差.
长非编码RNAs (lncRNAs) 在转移性肝母细胞瘤中的作用尚不清楚.

研究的目的:

调查lncRNA母体表达基因3 (MEG3) 在转移性肝细胞瘤中的作用.
为了确定MEG3表达是否与转移潜力相关.
评估MEG3在肝细胞母细胞瘤细胞行为中的功能意义.

主要方法:

RNA测序用于比较转移性肝细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞母细胞
定量PCR (qPCR) 用于验证MEG3表达在细胞系,瘤和患者衍生的异种移植.
通过siRNA介导的淘汰和过度表达实验来评估MEG3的 in vitro功能.

主要成果:

在转移性肝细胞母细胞瘤细胞和瘤中,MEG3显著上调.
降低MEG3降低了克隆原性,瘤球形成,迁移和入侵.
MEG3过度表达增强了迁移和入侵.

结论:

在肝细胞瘤中,MEG3促进了侵略性的瘤表型.
MEG3可以作为转移性肝母细胞瘤的潜在生物标志物或治疗点.
需要进一步的研究来阐明MEG3在肝细胞瘤进展中的机械作用.

关键词:

在MEG3中,MEG3是MEG3,MEG3是MEG3.肝炎母细胞瘤是什么在 lncRNA 中,转移转移转移转移

相关概念视频

02:39

lncRNA - Long Non-coding RNAs

10.0K

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

10.0K

02:39

lncRNA - Long Non-coding RNAs

3.8K

01:22

MicroRNAs

4.2K

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

4.2K

01:22

MicroRNAs

24.4K

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

24.4K

02:23

Abnormal Proliferation

5.3K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

5.3K