Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Risk Stratification of Adverse Outcomes After Heart Transplantation with the 2022 Definition of Pulmonary Hypertension.

Journal of cardiovascular development and disease·2026
Same author

Agonistic autoantibodies targeting AT1R and ETAR: Mechanistic insights and emerging implications in cardiovascular disease.

Journal of translational autoimmunity·2026
Same author

Autoantibody Profiling in Cardiomyopathies: Toward Immune-Guided Risk Stratification and Therapy.

Journal of clinical medicine·2026
Same author

Mortality reduction and survival extension in cardiovascular trials: why language matters.

Open heart·2026
Same author

From transplant rejection to coronary no-reflow: an immune-ischemic framework for antibody-mediated microvascular dysfunction.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Clinical Characteristics and Outcomes of Patients With Biventricular and Left-Dominant Arrhythmogenic Cardiomyopathy With Ring-like Late Gadolinium Enhancement Pattern.

JACC. Clinical electrophysiology·2026

相关实验视频

Updated: Feb 27, 2026

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.6K

一种新的基于心脏脱细胞化细胞外基质 (dECM) 的水凝:从其开发的标准化心肌脱细胞化程序到体外模型应用.

Giacomo Bernava1, Martina Boaron1, Golnar Abdalvand1

  • 1Cardiovascular Disease Modeling and Regenerative Medicine Group, Department of Cardiac Thoracic Vascular Sciences and Public Health, Padua Medical School, University of Padua, 35129 Padua, Italy.

Gels (Basel, Switzerland)
|February 26, 2026
PubMed
概括

研究人员用脱细胞化心脏组织制造了一种新的水凝. 这种生物相容的支架支持细胞生长,为心血管研究和药物测试提供了更好的体外模型.

关键词:
心脏组织工程心脏组织工程脱细胞化 脱细胞化这是一种水凝.在体外建模 in vitro 建模在心肌中,心肌.

更多相关视频

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

11.9K
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.5K

相关实验视频

Last Updated: Feb 27, 2026

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.6K
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

11.9K
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.5K

科学领域:

  • 生物材料科学 生物材料科学
  • 心血管研究研究心血管研究
  • 组织工程是组织工程.

背景情况:

  • 心血管疾病是全球主要的死亡原因.
  • 目前的体外模型 (2D培养) 缺乏复杂性,而体内模型有局限性.
  • 需要先进的体外模型来模拟本地心脏环境.

研究的目的:

  • 从脱细胞化猪心室肌肉 (dECM) 开发一种新的水凝支架.
  • 评估水凝的特性及其适用于心血管体外模型的适用性.
  • 评估dECM水凝在心脏组织工程和疾病建模中的潜力.

主要方法:

  • 优化了脱细胞化策略,以保护细胞外基质 (ECM) 蛋白质.
  • 基于dECM的水凝的自我交叉连接,凝和稳定性的特征.
  • 细胞兼容性测试使用人类骨髓衍生中酶体干细胞和人类静脉内皮细胞.

主要成果:

  • 该dECM水凝已成功制造,并保留了ECM结构.
  • 水凝表现出可重复的自我交叉连接,稳定的凝动力学和良好的稳定性.
  • 观察到高细胞活力和增殖,与2D相比,2.5D和3D培养中细胞密度增加.
  • 在dECM水凝有效支持心血管细胞培养在体外模型制造.

结论:

  • 基于dECM的心脏的水凝是一种结构保存和生物相容的平台.
  • 它支持短期和长期细胞培养,模仿本地心肌环境.
  • 这种支架在心脏组织工程,疾病建模和心脏毒性查方面具有重大潜力.