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相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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用于真菌转录组的功能注释工作流程

Nagisa Morihara1, Hidemasa Bono1,2

  • 1Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Hiroshima, Japan.

Journal of fungi (Basel, Switzerland)
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概括
此摘要是机器生成的。

这项研究引入了一个新的真菌特异性功能注释工作流程,用于RNA测序 (RNA-seq) 数据. 它使得快速,准确的转录分析,即使没有参考基因组,帮助真菌研究和应用.

关键词:
在RNA测序过程中,RNA测序全长的转录序列的测序.功能性注释功能性注释石竹是一种.大豆生的生.转录组的概况分析

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科学领域:

  • 菌类基因组学 菌类基因组学
  • 文字转录学 (Transcriptomics) 是一个学科.
  • 生物信息学是一种生物信息学.

背景情况:

  • 由于非模型物种的参考基因组有限,对真菌转录组的功能注释具有挑战性.
  • 现有的工具往往缺乏真菌特异性,需要参考基因组,阻碍了对新型真菌物种的分析.
  • RNA测序 (RNA-seq) 提供了快速的转录组概况,但需要强大的注释方法.

研究的目的:

  • 为RNA测序 (RNA-seq) 数据开发一种针对真菌的功能性注释工作流程.
  • 为了实现独立于参考基因组可用性的快速和准确的功能分析.
  • 与现有的注释工具相比,改进功能检测分辨率.

主要方法:

  • 开发一种针对真菌的标注工作流程.
  • 将工作流应用于RNA-seq和全长转录测序 (Iso-Seq) 数据,这些数据来自*Lentinula edodes* (石竹) 和*Phakopsora pachyrhizi* (亚洲大豆生).
  • 评价注释准确性和功能丰富检测.

主要成果:

  • 工作流成功注释了超过96%的蛋白质编码转录.
  • 已证明对RNA-seq和Iso-Seq数据的应用.
  • 实现了更高分辨率的功能检测,并确定了基因组编辑的潜在目标.

结论:

  • 开发的工作流程促进了真菌转录组的快速和准确的功能注释.
  • 它适用于各种真菌物种,包括没有参考基因组的真菌物种.
  • 工作流程有助于发现生物技术应用的功能性重要转录.