在Cordyceps militaris中温度调节的 Cordycepin 生物合成的分子机制
Jiaxing Shao1,2, Ziwei Zhang1,2, Guanhui Liu3
1Institute of Vegetables, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
Journal of fungi (Basel, Switzerland)
|February 26, 2026
概括
高温可通过调节生物合成和运输通路,显著提高Cordyceps militaris中的皮素生产. 这项研究阐明了这种关键化合物的温度控制积累的分子基础.
科学领域:
- 菌类学和生物化学 菌类学和生物化学
- 分子生物学和遗传学分子生物学和遗传学
- 代谢学和系统生物学
背景情况:
- 科尔迪塞是一种重要的生物活性化合物,由 *Cordyceps militaris* 生产.
- 确切的分子机制,控制温度受影响的cordycepin生物合成还没有完全理解.
- 优化皮质素产量需要对其调节途径有更深入的了解.
研究的目的:
- 为了阐明Cordyceps militaris*中温度调节的皮质素生物合成背后的分子机制.
- 为了研究不同温度 (15°C,20°C,25°C) 对 Cordycepin 积累的影响.
- 通过温度控制,为增强cordycepin生产提供理论基础.
主要方法:
- 综合的多omics方法,包括转录学和非目标代谢学.
- 权重基因共同表达网络分析 (WGCNA) 来识别关键的基因模块.
- 逆转录定量PCR (RT-qPCR) 用于验证基因表达模式.
主要成果:
- 在25°C的温度下,皮质素含量增加了84%,而在15°C的温度下,皮质素含量降低了15%.
- 温度显著影响膜功能,影响跨膜运输和脂肪酸代谢.
- 高温可以调高参与 purin代谢,酸通路和cordycepin生物合成 (Cns1-Cns3) 的基因,同时降低降解基因的调节并增强运输.
结论:
- 高温通过协同调节生物合成,降解和跨膜运输促进皮质素的积累.
- 这项研究确定了主要促进者超级家族 (MFS) 蛋白质对皮素运输具有潜在关键性.
- 这些发现为优化 *Cordyceps militaris* 种植以进行工业规模的 Cordycepin 生产提供了科学基础.
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