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Updated: Feb 27, 2026

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在Pichia pastoris中位置效应变异的全基因组范围的景观

Xueyan Yao^1,2, Baosong Chen³, Zhihui Zhang¹

¹State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Journal of fungi (Basel, Switzerland)

|February 26, 2026

概括

研究人员使用piggyBac转位子系统绘制了Pichia pastoris中的基因表达部位. 在基因内部发现了异性基因的高表达,这与染色质重塑途径有关.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

染色体整合是Pichia pastoris表达外来基因的标准.
对染色体位置影响和最佳表达部位的系统研究是有限的.

研究的目的:

使用piggyBac (PB) 转位子系统构建一个P. pastoris基因组随机插入库.
识别影响外源基因表达的基因位置.
为了阐明位置效应变化的全基因组范围的景观.

主要方法:

通过PB转位子系统构建P. pastoris基因组随机插入库.
确定PB识别站点 (TTAA) 以实现高效的基因组覆盖.
使用eGFP作为报告员,通过流细胞计隔离低表达和高表达图书馆.

主要成果:

确定TTAA序列是主要的PB识别站点.
在ORF的上游和终结器的下游发现了低表达性的网站.
高表达位点主要位于基因内部,与染色体重塑和基因素结合通路有关.
低表达位点与MAPK信号传递,自和阿金合成途径有关.

结论:

澄清了P. pastoris.中的全基因组位置效应变异景观.
确定基因内部有利于高异源基因表达.
为增强基因表达菌株的高通量查策略提供了见解.

关键词:

这就是Pichia pastoris.在 piggyBac 转位器上位置效应变化位置效应变化

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