Lactobacillus johnsonii DY2 从雅克斯分离 缓解急性大肠杆菌感染通过调节炎症反应,抗氧化能力和肠道微生物群
Yuhui Liu1, Yanlei Dong1, Muhammad Safdar2,3
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Veterinary sciences
|February 26, 2026
概括
来自雅克的 Lactobacillus johnsonii DY2 提供对大肠杆菌感染的保护. 这种益生菌减少炎症,增强抗氧化活性,加强肠道屏障,改善肠道微生物群平衡.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 抗菌素耐药性需要新的治疗策略.
- 益生菌被探索为替代抗生素的细菌感染.
- 乳杆菌种类显示出对抗感染的潜力.
研究的目的:
- 从雅克便中分离和鉴定Lactobacillus johnsonii DY2的特征.
- 在体内评估L. johnsonii DY2对急性大肠杆菌感染的保护功效.
- 研究L. johnsonii DY2的保护作用的潜在机制.
主要方法:
- 在体外抑制E.大肠杆菌的测定通过DY2超.
- 在体内研究使用小鼠口服预处理DY2在大肠杆菌挑战之前.
- 评估宿主免疫反应 (细胞因子,氧化应激标志物),肠道完整性 (组织学) 和肠道微生物群组成 (16S rRNA测序).
主要成果:
- DY2在体外抑制了大肠杆菌的生长.
- 用DY2治疗的小鼠显示体重减轻,细菌转移较低,器官指数正常化.
- DY2通过降低促炎细胞因子和氧化应激标志物来调节免疫反应,同时增加抗炎和抗氧化标志物.
- 肠道组织学显示,粘膜完整性得到保护,炎症减少.
- 微生物群分析显示,阿尔法多样性增加和有益细菌的丰富,如Butyricimonas.
结论:
- 牛乳杆菌 johnsonii DY2 显示出针对急性大肠杆菌感染的显著保护作用.
- 保护机制包括抗炎,抗氧化,增强肠道屏障和微生物群调节.
- 来自雅克的L. johnsonii菌株是益生菌开发的有希望的候选人,具有强大的抗菌特性.
关键词:
16S rRNA 测序的测序方法埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.牛乳杆菌约翰尼尼 (Lactobacillus johnsonii) 是一个雅克斯雅克斯是什么意思我们的肠道微生物群.益生菌是一种益生菌.更多相关视频
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