在尿液中检测SARM的综合IM-MS方法:建立CCS数据库以支持食品安全监测
Morgane Joly1,2, Bruno Le Bizec1, Fabrice Monteau1
1Oniris, INRAE, LABERCA, Nantes, France.
概括
选择性雄激素受体调节剂 (SARM) 在畜牧业中被滥用,尽管WADA禁止. 离子流动性光谱仪提供了准确的碰撞横截面 (CCS) 值,用于在反兴奋剂工作中可靠地检测SARM.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 法医科学 法医科学 法医科学
背景情况:
- 选择性雄激素受体调节剂 (SARMs) 是为了治疗目的而开发的,但现在已被世界反兴奋剂机构 (WADA) 禁止使用.
- 由于SARM很容易获得,因此人们担心它们在畜牧业中的非法使用.
- 不断发展的分析方法对于有效的反兴奋剂控制对SARM滥用至关重要.
研究的目的:
- 评估离子流动性 (IM) 谱法对于检测SARM的实用性.
- 使用不同的IM平台对SARM的碰撞横截面 (CCS) 值进行实验室间比较.
- 与预测工具相比,评估实验CCS测量的准确性.
主要方法:
- 一个实验室间比较,涉及30种不同的SARM的48个碰撞横截面 (CCS) 值.
- 使用了两个离子移动 (IM) 平台:移动波离子移动 (TWIMS) 和被困离子移动 (TIMS).
- 在一项案例研究中,分析了用Ostarine治疗的牛的尿样.
主要成果:
- 双胞胎显示0.40%的最大变化,TIMS显示0.34%的CCS值,这两个都低于2%的建议标准.
- 在实验室间比较中,92%的分析离子的偏差低于2%.
- 实验性CCS测量显示出明显高于CCS预测工具的准确性.
结论:
- 离子移动性 (IM) 光谱学为SARM检测提供了增强的选择性和灵敏性.
- 实验性CCS测量对于准确的SARM识别至关重要,其性能优于预测工具.
- IM提供了一个额外的参数 (CCS),可以提高对抗兴奋剂分析中的分析物识别和信号质量的信心.
更多相关视频
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
10.4K
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
1.5K
相关概念视频
High-Resolution Mass Spectrometry (HRMS)
2.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.6K
MALDI-TOF Mass Spectrometry
5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
Peptide Identification Using Tandem Mass Spectrometry
6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Mass Spectrometry: Complex Analysis
2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
High-Performance Liquid Chromatography: Types of Detectors
2.3K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
2.3K
