肠道病毒基因衍生细菌子内素Lys572可以有效控制冷藏和解的猪肉中的Streptococcus suis
Hui Ning1, Yiming Ma1, Siyao Zuo1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
International journal of food microbiology
|February 26, 2026
概括
一种新型的菌体内素,Lys572,有效地对抗猪肉中的Streptococcus suis污染. 这种酶在减少冷藏和解猪肉产品中的细菌方面表现出稳定性和有效性,提高了食品安全.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 瑞士杆菌 (S. suis) 是猪肉生产中的一个重要的动物传染病原体,污染了肉类和加工环境.
- 病毒组分析提供了一种培养独立的方法,用于发现新的抗菌蛋白质,如菌体内素,用于食品安全应用.
研究的目的:
- 鉴定和描述一种来自猪肠道病毒体的新型内素,该新型内素对S. suis.具有抗菌活性.
- 评估已识别的内素Lys572在控制猪肉产品中S. suis污染的有效性.
主要方法:
- 使用元基因组学分析从猪病毒体中识别内素.
- 新型内素Lys572的生物化学特征,包括其域结构和稳定性.
- 在体外评估Lys572对S. suis和S. equi的溶解活性,以及对S. suis生物膜的影响.
- 评估Lys572在降低猪肉中的S. suis在制冷和解过程中的有效性.
主要成果:
- 一种新的模块化内分泌素,Lys572被确定,具有CHAP,Amidase-2和ZoocinA_TRD域.
- 莱斯572在广泛的温度范围 (-80至45°C) 和pH值 (3.0至10.0) 中表现出稳定性,并且不受NaCl度的影响.
- 莱斯572在S. suis和S. equi的体外表现出强大的抗菌活性,并有效地根除了成熟的S. suis生物膜.
- 在48小时的冷藏过程中,Lys572显著降低了猪肉中的S. suis水平,约为2 log CFU/mL,在解过程中降低了2.0-2.2 log CFU/mL.
结论:
- 莱斯572是一种稳定且强效的内溶酶,具有广泛的抗菌活性,可以对抗相关的食源性病原体.
- 作为生物控制剂,Lys572具有显著的潜力,可以减少猪肉产品中的S. suis污染,改善食品安全和生物保存.
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