脂肪酸盐通过调节细菌运动性和抗生素吸收来促进抗生素耐药性的发展
Jinxian Yu1, Hanqing Wang2, Huijie Lu3
1State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou, Zhejiang 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China.
Journal of hazardous materials
|February 26, 2026
概括
脂肪酸盐 (FA) 在废水处理厂显著增加了抗生素耐药性. 这些非选择性化合物促进抗生素耐药性的发展,如cefepime,在各种环境中构成风险.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 废水处理技术 废水处理技术
背景情况:
- 废水处理厂 (WWTP) 是抗生素耐药性的公认热点.
- 抗生素耐药性是由抗生素驱动的,并可能由有机物等非选择性污染物恶化.
- 自然和外部来源的脂肪酸盐 (FA) 在WWTP中常见,对抗药性发展的影响尚不清楚.
研究的目的:
- 研究典型的FA对活性污泥 (AS) 中抗生素耐药性发展的影响.
- 在不同的条件下确定FA对抗塞费皮姆 (CPM) 的影响.
- 阐明FA促进抗生素耐药性的机制.
主要方法:
- 在AS暴露于有或没有FA (酸盐-C2,酸盐-C10,酸盐-C18) 的塞菲皮姆 (CPM) 10天.
- 在有氧和无氧条件下测量表型抵抗CPM的测量.
- 对抗生素耐药性基因 (ARG) 丰度和细菌物种隔离的分析,以进行进一步的测试.
主要成果:
- 在有氧 (35.9%-93.0%) 和无氧 (53.8%-92.3%) 条件下,与单独使用CPM相比,FA添加 (1 mg/L) 显著增加了对CPM的表型耐药性.
- 在有氧条件下,二甲酸显示了ARG丰度 (16.1%) 的最显著丰富.
- 在六种孤立的细菌物种中,FA促进了耐药性的发展,其中二甲酸盐在40天内显著增加了大肠杆菌的CPM耐药性的11.7倍.
- 脂肪酸降低了细胞移动性和EPS,同时增加了膜透性,增强了抗生素吸收和选择压力.
- 对其他抗生素 (西普洛克萨,四环素) 和更广泛的FA度范围 (1-100 mg/L) 观察到促进耐药性的影响.
结论:
- 像FA这样的非选择性物质在推动WWTPs抗生素耐药性发展方面发挥着重要作用.
- 脂肪酸可以通过涉及改变细菌生理学和增加抗生素选择压力的机制加剧抗生素耐药性.
- 研究结果强调了FA在促进各种环境中抗生素耐药性的风险.
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