相关实验视频

Updated: Feb 28, 2026

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

核斑使得从富含GC的同位体中处理RNA成为可能

Michał Małszycki¹, Lisa Martina¹, İbrahim Avşar Ilık¹

¹Max Planck Institute for Molecular Genetics, Berlin, Germany.

|February 26, 2026

概括

对于基因拼接至关重要的核斑点,通过去除核心组件SON和SRRM2.2来研究. 它们的缺失损害了富含GC的基因的拼接,突出显示了斑点在脊椎动物基因组进化中的作用.

科学领域:

细胞生物学细胞生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

核斑是重要的无膜有机体,参与转录后RNA处理.
SON和SRRM2是核斑点的核心组成部分,具有内在无序区域 (IDR).

研究的目的:

通过分析SON和SRRM2去除的影响来研究人类细胞中核斑点的功能.
探索核斑点,富含GC的同位体和脊椎动物的基因拼接之间的进化关系.

主要方法:

在人体细胞中工程性急性去除SON和SRRM2蛋白质.
对基因表达和拼接模式的分析,专注于富含GC的基因集群.
在真核生物之间进行比较基因组分析,以确定斑点和富含GC的同位体的进化出现.

主要成果:

删除SON和SRRM2导致GC丰富基因的显著下调,在GC丰富的同位体内具有短内子的GC丰富基因由于不高效的拼接.
富含GC的同位体外的基因不受影响,这表明斑点在这些序列的拼接中发挥了特定的作用.
核斑和富含GC的同位体仅在羊动物中发现,在脊椎动物中SON的IDR显著扩大.

结论:

核斑点对于高效地拼接富含GC的基因至关重要,特别是在胚胎动物中.
在SON中内在无序区域的演变可能促进了脊椎动物基因组中GC含量的增加.
这些发现揭示了核斑点功能,IDR扩张和脊椎动物基因组的结构组织之间的共同进化联系.

关键词:

通过RNA拼接进行RNA拼接.生物凝结物是一种生物凝结物.基因组的演化基因组的演化核组织核组织核光斑是核光斑的一种.

更多相关视频

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

相关概念视频

Additional Subnuclear Structures

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Export of mRNA

Nuclear Export of mRNA

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...