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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Riboswitches01:56

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Types of RNA01:23

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Types of RNA01:20

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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相关实验视频

Updated: Feb 28, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

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米Cis-Natural反感转录NAT1850的Pri-miR1850通过抑制NPR3负面调节冷耐受性

Yang Shen1,2, Yan Wang1, Lijia Yang3

  • 1Crop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.

Plant biotechnology journal
|February 26, 2026
PubMed
概括

自然的反感性转录 (NAT) 和小干扰RNA (siRNA) 调节了米的耐寒性和产量. 这项研究揭示了一种涉及pri-miRNA及其cis-NAT的新型调节机制,影响植物应激反应和农业生产力.

关键词:
在NPR3中,NPR3是NPR3.miR185050 的时间.如果是 siR185050寒冷耐受性 耐寒性 耐寒性谷物产量 谷物产量 谷物产量自然的反感性转录米米饭 米饭 米饭 米饭.

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 遗传学和基因组学 遗传学和基因组学
  • 农业科学 农业科学

背景情况:

  • 天然反感转录 (NAT) 在大米中很普遍,但它们的功能在很大程度上仍然没有特征.
  • 了解NAT对于提高大米的弹性和产量至关重要.

研究的目的:

  • 描述一个与pri-miR1850.0.重叠的水cis-NAT (NAT1850) 的特征.
  • 阐明NAT1850及其衍生的siRNA (siR1850) 对大米耐寒性和产量的调节作用.

主要方法:

  • 对转录组进行分析.
  • 它们是RNA干扰 (RNAi) 技术.
  • 基因表达分析 基因表达分析
  • 遗传学研究 遗传学研究

主要成果:

  • NAT1850和siR1850对大米的寒冷耐受性产生负面调节.
  • siR1850的目标是NPR3,这是冷应力信号通路的组成部分,涉及WRKY76和DREB1B.
  • NAT1850和siR1850也通过独立的途径影响的同化和产量.

结论:

  • 发现了一种新型的调节模块,涉及到一个pri-miRNA及其cis-NAT (NAT1850-siR1850).
  • 该模块在平衡冷应激反应和大米产量方面发挥着双重作用.
  • 研究结果提供了关于pri-miRNA调节及其对植物发育和应激适应的影响的见解.