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专门的阿尔多基因减解酶触发了真菌毒素脱氧尼瓦伦的完全降解

Weijie He¹, Renyi Xiong², Mengru Zheng¹

¹College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.

Nature communications

|February 26, 2026

概括

研究人员发现了一种新的细菌途径,用于完全降解脱氧尼瓦伦醇 (DON). 这种涉及C3-表皮化和C8-减少的酶过程,为控制食品和料中菌毒素污染提供了一个有前途的策略.

科学领域:

微生物学微生物学
生物化学生物化学
环境科学环境科学

背景情况:

脱氧尼瓦伦醇 (DON) 是一种普遍存在的真菌毒素,对人类和动物健康构成重大风险.
酶降解是排毒DON最有效的方法,但完整的降解途径仍然难以捉摸.

研究的目的:

阐明Nocardioides sp.中脱氧瓦伦醇降解的完整酶途径. 在S5-5.5中,它是S5-5.5.
识别和描述参与DON排毒的关键酶.

主要方法:

在Nocardioides sp.中的代谢途径分析在S5-5.5中,它是S5-5.5.
艾尔多基因减少酶 (DONepi和DONrd) 的基因鉴定和特征.
酶分析测试以确定基质特异性和催化机制.

主要成果:

确定了一种新型的代谢途径,涉及C3-epimerization和C8-reduction,用于完全降解DON.
两种水平转移的阿尔多基因还原酶基因DONEpi和DONrd被发现可催化这些关键步骤.
在C8位置上,DONepi执行立体逆转C3化,而DONrd则减少DON和3-epi-DON.
植物中DONepi的转基因表达赋予了对DON的耐药性,表明了作物保护的潜力.

结论:

已确定的酶途径为DON降解提供了一个完整的机制.
DONepi和DONrd是DON排毒的关键酶,为生物技术应用提供了潜在的目标.
使用DONEpi的工程工厂证明了在农业中减轻真菌毒素污染的可行策略.

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