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在雄性小鼠棕色脂肪细胞中,Bmal1通过调节脂质和OXPHOS基因表达来调节发热功能

Naoto Nagata¹, Ryuya Sugiyama¹, Susumu Kohno²

¹Department of Cellular and Molecular Function Analysis, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.

|February 27, 2026

概括

棕色脂肪细胞中的昼夜时钟对于能量消耗至关重要. 破坏这个时钟会损害脂肪分解和线粒体功能,可能导致代谢障碍.

科学领域:

循环节生物学日间生物
代谢生理学代谢生理学
棕色脂肪组织生物学

背景情况:

昼夜时钟调节了全身能量代谢.
棕色脂肪组织 (BAT) 对于不发的热生成和能量消耗至关重要.
在热生成中,BAT昼夜时钟的细胞自主作用需要进一步阐明.

研究的目的:

研究棕色脂肪细胞昼夜时钟在调节发热过程中的细胞自主功能.
为了确定昼夜时钟中断对棕色脂肪细胞代谢活动的影响.

主要方法:

主要棕色脂肪细胞与小鼠的BAT进行了区分.
通过使用Bmal1 siRNA敲击,创建了一个体外生物钟干扰模型.
通过测量氧气消耗率 (OCR) 来评估热能功能.
脂解酶和线粒体氧化酸化 (OXPHOS) 子单元的蛋白质水平通过西式涂抹分析.

主要成果:

Bmal1倒置显著降低了基底和刺激的OCR,表明热生成受损.
脂解酶 (ATGL,HSL) 和OXPHOS亚单元显示Bmal1缺乏细胞中的蛋白质表达减少.
尽管热生成受损,但细胞分化和β-上腺素反应能力被保留,UCP1表达升高.

结论:

棕色脂肪细胞的昼夜时钟通过调节脂质调动和线粒体氧化能力,对发热功能至关重要.
破坏BAT昼夜时钟会损害能源消耗,并可能增加对代谢障碍的易感性.
这些发现凸显了周围生态时钟在维持新陈代谢平衡中的重要性.

关键词:

棕色脂肪细胞棕色脂肪细胞循环节的时钟是循环节的时间.脂质溶解脂质溶解是一种奥克斯福斯 (OXPHOS) 是一个热生成是一种热生成.

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