相关实验视频

Updated: Feb 28, 2026

Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method

Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method

Published on: July 13, 2022

单细胞桥梁的乳酸驱动的重编程炎症性并发症中的骨损失

Junbin Wei¹, Zhiqian Ye¹, Deqian Tang²

¹Hospital of Stomatology, Guanghua School of Stomatology, South China Center of Craniofacial Stem Cell Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.

|February 27, 2026

概括

血中乳酸酸的升高与牙周炎和类风湿性关节炎中的炎症和骨损失有关. 关键基因SAT1,TET2和HIF1A可能作为这些炎症性骨疾病的生物标志物.

科学领域:

免疫代谢过程中的免疫代谢.
骨生物学骨生物学骨生物学
慢性炎症疾病慢性炎症疾病

背景情况:

炎症性骨损失在牙周炎 (PD) 和类风湿性关节炎 (RA) 中很常见.
这些不同的疾病共享免疫介导的骨再吸收机制.
了解分子驱动因素对于治疗并发性炎症性骨损失至关重要.

研究的目的:

在并发性炎症疾病中识别骨损伤的分子驱动因素.
调查乳酸代谢在PD和RA病变发生过程中的作用.
寻找潜在的生物标志物用于与炎症相关的骨损失.

主要方法:

使用PD和RA模型进行生物信息分析和实验验证.
单细胞RNA对PD和RA队列的测序.
机器学习来识别与乳酸相关的核心基因 (SAT1,TET2,HIF1A).
在体内和体外功能测定.

主要成果:

血中乳酸盐水平升高与PD和RA模型中的疾病严重程度相关.
乳酸代谢基因在单细胞中被上调,与炎症和骨质细胞生成有关.
SAT1,TET2和HIF1A显示出这两种疾病的诊断潜力.
乳酸重新编程单细胞,将免疫激活与骨再吸收联系起来.

结论:

乳酸驱动的免疫代谢轴连接免疫反应和骨重塑.
SAT1,TET2和HIF1A被确定为与炎症相关的骨损失的潜在生物标志物.
向乳酸代谢可能为并发性炎症性骨病提供治疗策略.

关键词:

炎症性骨损失导致的乳酸代谢乳酸代谢机器学习是机器学习.牙周炎是指牙周炎的一种疾病.类风湿性关节炎类风湿性关节炎一个单细胞RNA测序.

更多相关视频

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

相关概念视频

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.