分子信息学,化学测量和感官奥米克用于构建Umami类群库,遍及整个 Lager酒造过程
Yashuai Wu1, Ruiyang Yin2, Wenjing Tian3
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Foods (Basel, Switzerland)
|February 27, 2026
概括
这项研究确定了 lager 酒中关键的 umami ,揭示了它们在味道发展和感官感知中的关键作用. 了解这些酸有助于控制和预测酒的风味特征.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 感官科学 感官科学
背景情况:
- 乌玛米味道显著影响酒的体质,后味和整体口味表达.
- 了解酒的乌玛米口味的分子基础对于口味控制和发展至关重要.
研究的目的:
- 在整个酒过程中识别和表征乌玛米.
- 阐明乌玛米与T1R1/T1R3受体相互作用的分子机制.
- 为了将乌玛米的丰富度与对酒乌玛米味道的感官感知相关联.
主要方法:
- 使用RPLC-QTOF-MS进行阶段性皮体样本分析.
- 使用UMPred-FRL和TastePeptides-Meta. 的生物信息查.
- 用T1R1/T1R3.3.进行乌玛米的分子对接和结构分析.
- 通过将酸注入酒样本进行感官验证.
主要成果:
- 在25,592个总中鉴定了7255个潜在的乌玛米.
- 阐明了在T1R1/T1R3受体中涉及离子和疏水相互作用的协同结合机制.
- 证明仅仅结合亲和力并不能预测感知的乌玛米;较长的可以对味道产生负面影响.
- 在累积的乌玛米丰度和感官乌玛米得分之间发现了强烈的正相关性 (r=0.963).
结论:
- 乌玛米是 lager 酒风味的关键决定因素,它们的累积丰度与感知到的乌玛米味道直接相关.
- 为了过程控制和风味预测,建立了一个新的乌玛米类集群数据库.
- 这项研究为了解和操纵发酵饮料中的乌玛米味道提供了一个框架.
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