乳酸细菌的抑制机制 无细胞的超级生物对储存的谷物菌
Can Cui1, Xiaopeng Fu1, Tianjie Qi1
1School of Food and Strategic Reserves, Henan University of Technology, Zhengzhou 450001, China.
Foods (Basel, Switzerland)
|February 27, 2026
概括
乳酸细菌 (LAB) 显示出抑制谷物中储存模具的潜力. 它们的无细胞超,富含有机酸如酸和乳酸,有效抑制真菌生长,提供一种天然的保存方法.
科学领域:
- 食品微生物学 食品微生物学
- 农业科学 农业科学
- 生物技术是生物技术.
背景情况:
- 谷物被真菌破坏会造成重大经济损失和质量下降.
- 乳酸细菌 (LAB) 因其对储存的谷物模具的抗真菌特性而受到研究.
- 有限的LAB菌株及其抑制机制的理解阻碍了应用.
研究的目的:
- 隔离并识别具有抗真菌活性的LAB菌株,以对抗常见谷物菌.
- 研究LAB的抗真菌机制,重点关注有机酸的作用.
- 确定LAB生产的特定有机酸对关键真菌物种的有效性.
主要方法:
- 使用双层板测试对71个LAB菌株进行隔离和选,对*A. flavus*,*A. niger*和*P. citrinum*进行检测.
- 使用形态特征和16S rRNA基因测序识别有前途的LAB菌株.
- 通过pH调整,酶处理,HPLC和直接抗真菌测定,对LAB无细胞超 (CFS) 组成和抗真菌活性进行分析.
主要成果:
- 17种LAB菌株表现出抑制活性;被确定为*Weissella cibaria*,*Pediococcus pentosaceus*和*Lactiplantibacillus paraplantarum*.这些菌株具有抑制作用.
- 在LAB CFS中,有机酸被证实是主要的抗真菌剂.
- 鉴定了酸,酸,乳酸,酸和酸;酸和乳酸对*P. citrinum*和*A. flavus*最有效,而酸和酸则抑制了*A. niger*.
结论:
- 实验室,特别是确定的菌株,可以有效地抑制有害的谷物储存真菌的生长.
- 由LAB产生的有机酸是抗真菌活性的关键介质.
- 这些发现为利用LAB无细胞超级体作为天然谷物防腐剂提供了科学依据.
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