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从商业益生菌中分离,识别和验证菌株:我们得到了我们期望的吗?

Isabella Somera de Oliveira E Silva^1,2, Emília Maria França Lima¹, Katia Leani¹

¹ProBacLab, Laboratório de Microbiologia de Alimentos, Departamento de Alimentos e Nutrição Experimental, Food Research Center, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.

Foods (Basel, Switzerland)

|February 27, 2026

概括

许多商业益生菌产品与Limosilactobacillus reuteri.的标签声明不符. 严格的菌株鉴定和质量控制对于确保益生菌的安全性和有效性至关重要.

科学领域:

微生物学微生物学
益生菌益生菌益生菌
食品科学食品科学食品科学

背景情况:

商业益生菌产品广泛可用,通常声称具有特定的微生物含量,如Limosilactobacillus reuteri.
确保商业产品中的益生菌微生物的生命力,身份和安全性对于消费者健康和监管合规性至关重要.

研究的目的:

为了评估含有Limosilactobacillus reuteri的益生菌产品的微生物质量,功能特征和安全性.
评估标签声明关于可活细胞数量和微生物组成的准确性.
为了进行菌株级别的识别,并描述孤立微生物的功能和安全属性.

主要方法:

评估活跃细胞数量 (殖民地形成单位) 并对分离物进行生物化学表征.
使用rep-PCR和16SrRNA测序用于菌株级别的识别和微生物组成分析.
进行了蛋白质和二甲基生成能力,胃肠道耐受性,乳酸生产,抗生素敏感性的功能测定,并查了毒性标志物和污染物.

主要成果:

在标签声明和实际微生物含量之间发现了显著的差异;在12种产品中,只有4种符合殖民地形成单位要求,而2种缺乏活性的微生物.
分子鉴定发现了Limosilactobacillus reuteri,Lacticaseibacillus rhamnosus和其他物种的混合物,如Lactiplantibacillus plantarum和Lactobacillus acidophilus.
菌株之间的功能性质有显著差异,所有分离物对推的抗生素都敏感,没有检测到可转移的毒性因子或污染物.

结论:

商业益生菌产品在质量上表现出相当大的差异,许多产品未能满足Limosilactobacillus reuteri的标签要求.
菌株级特征是验证益生菌微生物的身份,安全性和功能性能不可或缺的.
这些发现强调了益生菌行业需要严格的质量控制措施,以确保产品的完整性和遵守法规.

关键词:

D-乳酸盐的使用方法在GITIT的基础上,GITIT是GITIT.牛奶的乳酸酶ibacillus rhamnosusus是什么意思利莫西拉克托巴西勒斯 (Limosilactobacillus reuteri) 是一种植物.迪亚酸乙烯 (Diacetyl) 是一种乳酸细菌是一种乳酸细菌.

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