尼古丁胺胺单核酸通过NR4A1和犬肠表皮细胞中的海斯修饰来调节内素-1
Xudong Guo1,2, Chuyang Zhu2, Saber Y Adam2,3
1College of Physical Education and Health, Jiangsu Vocational Institute of Commerce, Nanjing 211168, China.
Animals : an open access journal from MDPI
|February 27, 2026
概括
尼古丁胺胺 mononucleotide (NMN) 显著影响狗肠道上皮细胞的增殖和炎症通路. NMN治疗可以提高细胞能量和抗氧化剂水平,这表明对狗肠道健康的潜在益处.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 尼古丁胺胺单核酸 (NMN) 是NAD+的前体,以抗衰老和抗炎性质而闻名.
- 肠上皮细胞 (cIECs) 在肠道健康和屏障功能中起着至关重要的作用.
- 了解NMN对CIEC的影响可以揭示肠道疾病的治疗潜力.
研究的目的:
- 在转录基层研究NMN对犬肠上皮细胞 (cIECs) 的影响.
- 阐明NMN对cIEC细胞增殖,衰老和炎症的影响背后的分子机制.
- 评估NMN对细胞能量代谢和cIEC中氧化应激标志物的影响.
主要方法:
- 用NMN治疗的CIECs的转录组分析与对照组相比.
- 测量细胞中的ATP,SOD,CAT,GSH,ROS和MDA水平.
- 通过ChIP-qPCR分析线粒体复合酶活动和基因/激素修饰表达.
主要成果:
- NMN显著影响了细胞增殖,并丰富了细胞周期,衰老和炎症通路中的基因.
- NMN治疗增加了ATP,SOD,CAT,GSH水平,并降低了ROS,MDA活动,增强了线粒体功能.
- NMN调节了关键基因 (如MAPK13,EDN1,NR4A1) 和基因素标记物 (H3K23ac,H3K27ac,H3K27me3) 的表达,通过NR4A1.3影响EDN-1调节.
结论:
- NMN显示出显著的能力调节生物过程在CIECs,包括增殖和炎症反应.
- NMN增强细胞能量代谢和抗氧化剂防御,可能改善肠道健康.
- NMN通过NR4A1和基因素修饰来调节EDN-1表达,这表明它对狗肠道健康的治疗作用具有新的机制.
更多相关视频
08:24Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
Published on: November 18, 2016
11.8K
08:08Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta
Published on: June 10, 2015
11.9K
相关概念视频
Regulation of Angiogenesis and Blood Supply
3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
