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与牛奶脂肪代谢相关的ceRNA调控网络的构建

Xiaofang Feng^1,2, Shenglai Cheng¹, Zhiyu Lu¹

¹School of Life Sciences and Engineering, Northwest Minzu University, Lanzhou 730030, China.

Animals : an open access journal from MDPI

|February 27, 2026

概括

这项研究揭示了关键的循环RNAs (circRNAs) 和微RNAs (miRNAs) 调节牛奶脂肪. 了解这些竞争的内源性RNA (ceRNA) 网络为提高奶牛牛奶质提供了一条途径.

科学领域:

* 分子生物学 * 分子生物学
* 动物科学动物科学
* 基因组学是一个学科.

背景情况:

* 牛奶脂肪百分比 (MFP) 显著影响牛奶的营养价值,风味和味道.
* 非编码RNA在调节牛奶脂肪代谢中起着至关重要的作用.
*确定监管机制对于改善奶牛的牛奶质量至关重要.

研究的目的:

* 为了研究牛乳腺上皮细胞 (BMECs) 中特定circRNAs的调控作用,具有不同的MFP.
* 构建和分析参与牛奶脂肪代谢的竞争性内源性RNA (ceRNA) 调节网络.
*验证ceRNA网络中的相互作用,阐明关键miRNAs的功能.

主要方法:

*在高和低MFP的BMEC中对circRNAs的差异表达分析.
* RNase R消化,桑格测序和亚细胞局部化以表征circRNAs.
*使用Cytoscape和生物信息学工具构建一个ceRNA网络.
*定量实时PCR (RT-qPCR),双光酶记者基因测定,以及miRNAs的功能验证.

主要成果:

* 在BMEC中确定了四种不同表达的circRNAs (上调:circ_0009058,circ_0004021;下调:circ_0011934,circ_0008056).
* 构建了一个具有六个枢纽基因 (VAV1,PTPN6,PIK3R1,RHOA,ERBB3,PIK3CG) 的ceRNA网络.
*已验证的关键相互作用:circ_0004021/bta-miR-541/PTPN6,circ_0008056/bta-miR-2309/ERBB3,以及bta-miR-10175-3p/RHOA. 这两种关键相互作用均已得到验证.
* 证明bta-miR-541和bta-miR-10175-3p可以降低脂质水平,而bta-miR-2309则具有相反的效果.

结论:

*确定了新的circRNA-miRNA-mRNA ceRNA网络,调节奶牛的牛奶脂肪代谢.
*这些发现为提高牛奶脂肪产量和质量的有针对性的战略提供了理论基础.
*这项研究阐明了乳脂合成背后的分子机制,并为遗传改进提供了潜在的目标.

关键词:

环RNA 环RNA 是一个环RNA.具有竞争力的内源性RNA.奶牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛哺乳动物上皮细胞牛奶的脂肪和牛奶的脂肪.

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