自然杀手细胞的表型和功能作为预测因素治疗反应的新辅助疗法在乳腺癌患者的治疗反应
Cinthya Yareli Anguiano Serrato1, Fabiola Solorzano-Ibarra2, Ignacio Mariscal-Ramirez3
1Hospital General Regional N°2 El Marqués, Instituto Mexicano del Seguro Social, Querétaro 76246, Querétaro, Mexico.
International journal of molecular sciences
|February 27, 2026
概括
自然杀手 (NK) 细胞预测乳腺癌 (BC) 患者接受新辅助全身疗法 (NST) 的治疗反应. 增强的NK细胞活性和激活受体与病理完整反应 (pCR) 相相关,表明NK细胞表型作为生物标志物.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 流动细胞计量 (Flow Cytometry) 是一种流动细胞计.
背景情况:
- 新辅助系统疗法 (NST) 是局部晚期乳腺癌 (BC) 的标准,但需要病理完整反应 (pCR) 的预测剂.
- 自然杀手 (NK) 细胞对于抗瘤免疫是至关重要的,但它们在BC的NST期间的作用和受体动态尚不清楚.
研究的目的:
- 描述与接受NST的BC患者治疗成功相关的外围NK细胞格局和免疫特征.
- 为了确定NK细胞表型和受体动态,预测pCR到NST在局部晚期乳腺癌.
主要方法:
- 在34名BC患者和35名健康捐赠者 (HD) 的前性队列研究中.
- 使用高参数流细胞计和UMAP对外围血液NK细胞子集的纵向评估,跟踪NST前后的表型 (NKG2D,DNAM-1,PD-1,TIGIT).
- 评估NK细胞细胞毒性和血清细胞因子/标记物水平 (例如IL-2,TNF-α,IFN-γ).
主要成果:
- 与非pCR患者相比,获得pCR (12/34) 的BC患者表现出改善的NK细胞细胞毒性和治疗后更高的IL-2,TNF-α,sFASL和Granzyme B水平.
- 非pCR患者在治疗后显示CD56明亮NK细胞的增加和细胞毒性CD56暗NK细胞的减少,以及抑制检查点 (TIGIT,PD-1) 的共同表达的增加.
- pCR患者表现出激活受体 (NKG2D,DNAM-1) 的更有利平衡以及有益的TIGIT/DNAM-1轴比率,表明NK细胞功能得到增强.
结论:
- 在接受NST的BC患者中,NK细胞表型和活性与治疗反应有关.
- 增强的NK细胞细胞毒性,更高的激活受体表达 (NKG2D,DNAM-1),以及有利的TIGIT/DNAM-1轴,是患者实现pCR的特征.
- NK细胞表型评估,特别是TIGIT/DNAM-1轴,可以作为乳腺癌中NST反应的预测生物标志物.
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