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Updated: Feb 28, 2026

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FlowCLOc,一种用于在流细胞计中选择最合适抗体的新工具

Valentina Serra¹, Valeria Orrù¹, Sandra Lai¹

¹Institute for Genetic and Biomedical Research, National Research Council, 08045 Lanusei, Italy.

International journal of molecular sciences

|February 27, 2026

概括

冷保存显著改变了在流细胞计中的免疫细胞标记物表达. 一个新的公共数据库,FlowCLOc,对这些变化进行了目录,以帮助选择可靠的冷和新鲜样本抗体.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

流细胞计对循环免疫细胞的免疫类型定型至关重要.
样品的冷保存可以影响细胞恢复和标记物表达,可能影响实验结果.

研究的目的:

系统地评估冷保存对各种细胞表面标记物的表达的影响.
开发一种资源,以优化新鲜和冷样品的流量细胞计面板设计.

主要方法:

从新鲜的B,T,NK丰富细胞和单细胞对438个抗体进行比较,与冷保存的淋巴单细胞和血液对比.
标记物表达变化超过20%的定量评估.

主要成果:

冷保存显著改变了淋巴单细胞中的283个标记物的表达和血液中的262个标记物的表达.
很大一部分测量标记物在冷保存后显示>20%的变化.

结论:

在使用冷样本进行流细胞计时,对抗体性能进行仔细评估是必不可少的.
FlowCLOc数据库为简化面板设计和提高冷和新鲜样品数据可靠性提供了有价值的工具.

关键词:

流量CLOccc 的时间.这就是PBMCs.冷保存的方法流动细胞计量是流动细胞计量的方法.冰的血液,冷的血液.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...