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相关概念视频

RNA Interference01:23

RNA Interference

28.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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相关实验视频

Updated: Feb 28, 2026

mRNA Interactome Capture from Plant Protoplasts
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mRNA Interactome Capture from Plant Protoplasts

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编码转录衍生小干扰RNA:它们在Arabidopsis中的生物发生和分子功能

Xintong Xu1, Nier Chen1, Xinwen Qing1

  • 1Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

International journal of molecular sciences
|February 27, 2026
PubMed
概括
此摘要是机器生成的。

编码转录衍生的小干扰RNAs (ct-siRNAs) 在RNA质量控制受损时产生,影响基因表达和植物生长. 本综述探讨了ct-siRNA在植物中的生物发生,功能和后果.

关键词:
DCL2 DCL2 的时间DCL4 DCL4 的时间EIN5 / XRN4 的使用情况.RDR6 RDR6 是一个在RQC中,RQC就是RQC.美国SGS3在SKI2中,SKI2是SKI2.ct-siRNAs 的存在.

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 在RNA生物学,RNA生物学.
  • 遗传学 是一个遗传学.

背景情况:

  • 编码转录衍生小干扰RNAs (ct-siRNAs) 是内源小RNAs参与基因调节.
  • 它们的生物发生与RNA质量控制 (RQC) 系统和RNA指导的RNA聚合酶6 (RDR6) 有关.
  • 当RNA代谢途径受到干扰时,ct-siRNAs会积累,影响植物表型.

研究的目的:

  • 在各种RNA代谢缺陷条件下审查ct-siRNAs的生物发生.
  • 分析ct-siRNA作用的机制,包括翻译抑制和mRNA裂变.
  • 总结ct-siRNA积累对植物生长和应激反应的生物学后果.

主要方法:

  • 文献综述和基因背景的比较分析.
  • 检查siRNA长度和遗传背景对监管结果的影响.
  • 综合关于ct-siRNA功能和影响的当前研究.

主要成果:

  • 当RQC受损时,ct-siRNAs通过RDR6和DICER-LIKE蛋白质从异常的mRNA片段生成.
  • ct-siRNAs通过翻译抑制和/或mRNA分裂来调节基因表达.
  • 积累ct-siRNAs与植物生长受损和应激适应相关.

结论:

  • ct-siRNAs 作为缺陷RNA代谢和转录后基因沉默之间的联系.
  • ct-siRNAs的调节效应取决于上下文,受siRNA长度和遗传背景的影响.
  • 需要进一步的研究来解决关于ct-siRNAs的当前争议和未解决的问题.