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在使用的酒厂谷物中获得的碳基材料上的Laccase固定:优化和稳定性评估

Ângela Almeida¹, Marta Pereira², Ana Peleja¹

¹Department of Chemistry and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.

Molecules (Basel, Switzerland)

|February 27, 2026

概括

使用已耗尽的酒谷物生物炭 (SBG-BC) 进行酶固定,可以提高乳酸酶的稳定性和环境应用中的可重复使用性. 这种更绿色的方法显示了废水处理和监测的潜力.

科学领域:

生物技术是生物技术.
环境科学环境科学
材料科学材料科学材料科学

背景情况:

酶固定在固体支上可以提高稳定性,可重复使用性和效率.
乳糖酶 (Lac) 是各种工业和环境应用的关键酶.

研究的目的:

为了研究从Trametes versicolor*获得的乳糖酶 (Lac) 物理固定到从消耗的酒厂谷物 (SBG) 获得的活性炭 (AC) 和生物炭 (BC) 上.
评估SBG衍生碳材料的性能和可持续性,以此来支持乳固定.

主要方法:

通过微波热解,从废弃的酒厂谷物中生产活性炭 (SBG-AC) 和生物炭 (SBG-BC).
在不同的条件下 (pH,酶度) 将laccase物理固定到SBG-AC和SBG-BC上.
固定拉卡斯的表征,包括固定效率,最大活性和动力分析 (K_M).

主要成果:

在pH值为5.0的SBG-AC/Lac上,固定效率高达80%.
在pH3.5和40°C时,SBG-AC/Lac的最大活性为5.5U/g,SBG-BC/Lac的最大活性为4.6U/g.
与自由乳糖酶 (Lac_f) 相比,SBG-BC/Lac表现出更高的稳定性,在24小时后保持约60%的活性.

结论:

消耗的酒厂谷物生物炭 (SBG-BC) 是一种可持续和有效的支乳固定,提供增强的稳定性.
SBG-BC代表了化学活性炭的更绿色替代品,用于乳糖酶固定.
固定式乳酸盐在废水处理和环境监测中的应用具有前景.

关键词:

农业废物农业废物碳基材料是基于碳的材料.环境应用程序环境应用程序氧化还原酶可以减少氧化.身体不动化身体不动化

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