在试管婴儿中体积的模式和混乱的胚胎:根据年龄分层分析和来自罗马尼亚队列的再活检结果
Anca Huniadi1,2, Petronela Naghi2, Iona Zaha1
1Faculty of Medicine and Pharmacy, University of Oradea, 1st December Square 10, 410073 Oradea, Romania.
Medicina (Kaunas, Lithuania)
|February 27, 2026
概括
形积分症是试管婴儿失败的主要原因,随着母亲的年龄增加而增加. 在植入前遗传测试 (PGT-A) 中,混沌胚胎的再活检证实了它们的异常,突出了PGT-A.
科学领域:
- 生殖医学 生殖医学
- 遗传学 是一个遗传学.
- 胚胎学 胚胎学
背景情况:
- 积体是植入失败和流产的主要驱动因素,其发病率随着母亲的年龄显著增加.
- 由于错误分类的风险,胚胎中的混乱和复杂形状在植入前形状遗传检测 (PGT-A) 中存在诊断挑战.
研究的目的:
- 调查不同孕妇年龄组内积体和混乱胚胎的患病率.
- 评估再活检在澄清最初被诊断为混乱的胚胎状态中的实用性.
- 评估PGT-A作为诊断和质量控制工具的作用.
主要方法:
- 对来自罗马尼亚试管婴儿队列的230个胚胎细胞的回顾性分析,按母亲年龄 (25-50岁) 分层.
- 热囊细胞活检和下一代测序 (NGS) 用于染色体分析.
- 对19个最初混乱的胚胎进行重新活检,以评估诊断的准确性和一致性.
主要成果:
- 积体病的发病率从29.6% (25-30岁) 增加到68.7% (41-50岁;p=0.002). 积体病的发病率从29.6% (25-30岁) 增加到68.7% (41-50岁;p=0.002).
- 质量差的胚胎囊显示出较高的形积分率 (72.4%) 与质量好的胚胎相比 (34.6%;p=0.004).
- 混沌胚胎的再活检显示了持续的异常,这表明初始PGT-A结果的技术变异性,而不是真正的euploidy.
结论:
- 胚胎形积分发病率随着母亲年龄的增加而升级,这凸显了体外受精中与年龄相关的风险.
- 混乱的胚胎本质上是异常的;再活检有助于完善PGT-A的解释和质量控制.
- PGT-A作为一种至关重要的诊断工具,但其在识别euploid胚胎方面的局限性需要在临床实践中仔细考虑.
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