综合基因调节网络分析揭示了Arabidopsis thaliana-Trichoderma atroviride相互作用中的协调转录重编程
Evelyn Sánchez1,2, Lorena Melet1,2,3, José D Fernández1,2,3
1Centro de Genómica y Bioinformática, Universidad Mayor, Santiago 8580745, Chile.
这项研究揭示了Arabidopsis植物和Trichoderma真菌如何通过基因调节网络 (GRNs) 和小RNA (sRNAs) 协调生长. 了解这些植物-真菌相互作用有助于开发有益的农业应用.
科学领域:
- 植物与微生物的相互作用
- 菌生物学的真菌生物学
- 分子遗传学 分子遗传学
背景情况:
- 相互的植物-真菌相互作用涉及复杂的转录重编程.
- 管理这些共生的协调监管机制尚未完全理解.
研究的目的:
- 在*Arabidopsis thaliana*和*Trichoderma atroviride*之间的相互作用过程中剖析基因调节网络 (GRNs) 和小RNA (sRNA) 动态.
- 为理解支持植物真菌共生的协调法规提供系统级的框架.
主要方法:
- 转录组学分析以确定两种生物体中差异表达的基因.
- 基因调控网络 (GRN) 推断使用DNA结合动机和GENIE3.
- 分析小RNA (sRNA) 配置文件及其在双方合作伙伴中的潜在目标.
主要成果:
- 在注射后72小时观察到广泛的转录变化,无论是 * Arabidopsis * (低氧,压力,根部发育) 和 * T. atroviride * (膜传输,代谢).
- 重建的物种特异性GRNs揭示了协调关键过程的TF模块 (植物中的ERF,WRKY,NAC,DOF;真菌中的CrzA,RME1,NsdC,PacC,RPN4).
- 与之相反的sRNA动态:在*Arabidopsis*中变化有限,但在*T. atroviride*中具有强烈的诱导作用,其中一些sRNA准宿主免疫和根功能基因.
结论:
- 该研究提供了一个系统层面的理解监管程序,使 * Arabidopsis-Trichoderma * 互惠.
- 确定了协调宿主和真菌反应的关键转录因子和sRNA相互作用.
- 为未来研究有益于植物的微生物相互作用提供了可测试的框架.
更多相关视频
09:31Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Published on: April 19, 2019
09:41Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Published on: May 14, 2020
相关概念视频
Gene Regulation During Sporulation
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps
