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宿主细胞受体和疹简单病毒1 糖蛋白B 是低温进入的决定因素

Colleen M Lynch¹, McKenna A Hull¹, Anthony V Nicola¹

¹Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.

|February 27, 2026

概括

简单疹病毒1 (HSV-1) 进入机制复杂且取决于细胞类型. 这项研究确定了nectin-2和glycoprotein B (gB) 作为HSV-1低温进入的关键决定因素.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

简单疹病毒1 (HSV-1) 进入涉及复杂的病毒和宿主因子相互作用.
HSV-1的进入机制尚未完全理解,并且因细胞类型而异.
研究非典型的HSV-1菌株有助于识别进入的决定因素.

研究的目的:

通过HSV-1菌株 ANG路径调查低温 (4°C) 进入的决定因素.
阐明特定病毒蛋白和宿主受体在降低温度下HSV-1进入中的作用.
确定控制HSV-1低温进入的细胞类型特定机制.

主要方法:

使用了具有非典型入口特性的HSV-1菌株 (ANG路径,KOS,奇米拉27/III).
在4°C的温度下在各种细胞系 (Vero,CHO-K1,HaCaT) 中评估病毒的进入.
研究了特定受体 (nectin-1,nectin-2) 和病毒糖蛋白 (gB) 在输入中的作用.

主要成果:

在Vero细胞中,HSV-1菌株 ANG路径在4°C时呈现出进入,与野生型菌株不同.
尼克丁-2 在CHO-K1细胞中介于HSV-1 ANG路径的4°C进入,而尼克丁-1则没有.
HSV-1菌株KOS的糖蛋白B (gB) 被确定为低温进入HaCaT细胞的决定因素.

结论:

多种决定因素和机制有助于HSV-1低温进入.
在低温下进入HSV-1的要求是特定于细胞类型的.
像gD这样的受体结合蛋白和像gB这样的病毒糖蛋白在不同温度条件下调节HSV-1的进入中起着至关重要的作用.

关键词:

葡萄糖蛋白B是一种B型葡萄糖蛋白.简单疹病毒 1 简单疹病毒低温进入的入口.接收器接收器接收器接收器

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