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针对SARS-CoV-2主蛋白酶:一种基于细菌的色度测试,用于选天然抗病毒抑制剂

Shaza S Issa¹, Andrew A Zelinsky², Haidar J Fayoud^1,3

¹Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia.

|February 27, 2026

概括

开发了一种新的细菌色度测试法,用于选SARS-CoV-2主要蛋白酶 (Mpro) 抑制剂. 这种快速,经济有效和生物安全的方法有助于优先考虑抗病毒药物发现的天然化合物.

科学领域:

生物化学生物化学
微生物学微生物学
药物发现药物发现药物发现

背景情况:

SARS-CoV-2 主蛋白酶 (Mpro) 对于病毒复制至关重要,也是抗病毒疗法的关键标.
现有的Mpro抑制剂选方法缺乏功能上下文,昂贵,或需要高的生物安全水平.
需要简单,快速和生物安全的功能查工具,以优先考虑早期的抑制剂,特别是天然产品.

研究的目的:

开发一种新型的细菌色度测定记者试验,用于SARS-CoV-2 Mpro 抑制剂的功能查.
建立一个具有成本效益和生物安全性的平台,优先考虑来自自然来源的潜在Mpro抑制剂.

主要方法:

在*Escherichia coli*中设计了一种细菌试验,将SARS-CoV-2 Mpro活动与β-galactosidase功能联系起来.
该试验使用X-gal含有介质的色度读数用于可视检测蛋白酶抑制.
使用石榴汁和其他植物衍生制剂来证明概念的证明.

主要成果:

开发的测试提供了SARS-CoV-2 Mpro抑制的快速,视觉和表型读数.
该试验具有成本效益和生物安全性,适用于标准实验室条件.
已证明适用于各种自然基质,如石榴,大胡桃,葡萄和胡果汁.

结论:

这种细菌色度测试是首个用于功能检测SARS-CoV-2 Mpro抑制的细菌色度测试.
它作为一个有价值的工具,在进一步验证之前,对有前途的抑制剂候选者的早期优先考虑.
确认了该试验的细菌背景和化合物吸收限制,强调其在初始查中的作用.

关键词:

美国大肠杆菌 (E. coli).Mpro 抑制剂的使用这就是SARS-CoV-2病毒.颜色测量试验的测试方法子子是一种子.石榴果汁是什么意思?石榴果汁是什么意思拉巴巴是一种拉巴巴.选试验选试验选试验这就是β-galactosidase.

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