SARS-CoV-2主要通过非正规的进入途径感染人类初级细胞核细胞体
Hélène Pinatel1,2, Marie-Eve Brien3, Mathilde Broquière1
1Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique, Laval, QC, Canada.
Molecular human reproduction
|February 27, 2026
概括
人类胎盘细胞可以被SARS-CoV-2感染,这是导致COVID-19的病毒. 这项研究调查了病毒进入的途径,并发现胎盘细胞对各种菌株具有宽容性,这对理解怀孕结果有意义.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 产科和妇科 产科和妇科
背景情况:
- 在COVID-19大流行期间,孕妇是脆弱的人群.
- 母亲的SARS-CoV-2感染可能会导致胎盘变化.
- 热囊细胞对不同SARS-CoV-2变异的宽容性尚未得到充分理解.
研究的目的:
- 为了评估人类热囊细胞对SARS-CoV-2的宽容性.
- 为了描述病毒进入热囊细胞的途径.
- 评估热囊细胞系作为SARS-CoV-2感染的模型.
主要方法:
- 感染了原发性状细胞质细胞和质细胞细胞系 (BeWo,JEG-3,HIPEC-65) 与祖先 SARS-CoV-2 的感染.
- 使用RT-PCR,免疫染色和斑块检测评估病毒的容许性.
- 使用TMPRSS2和内体抑制剂检查病毒进入途径;对SARS-CoV-2变种进行测试.
主要成果:
- 主要状细胞核细胞体在体外允许所有测试的SARS-CoV-2菌株.
- 祖先的SARS-CoV-2主要通过非正规的内体路径进入热囊细胞.
- JEG-3细胞作为一个合适的模型,反映了主要细胞的允许性和进入机制.
结论:
- 细胞细胞对SARS-CoV-2的宽容性受病毒遗传因素的影响.
- 这项研究提供了对SARS-CoV-2在人体热囊细胞的进入机制的见解.
- 在怀孕期间,JEG-3细胞是研究 trofhoblast 感染和 SARS-CoV-2 发病因的有价值模型.
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