CELF1是一种非正规的eIF4E结合蛋白,促进表皮-介质细胞过渡效应 mRNAs 的翻译
Arindam Chaudhury1,2, Natee Kongchan1,2, Shebna A Massey1,2
1Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, United States.
Nucleic acids research
|February 27, 2026
概括
CELF1蛋白与eIF4E和PABPC1结合,促进特定mRNA的翻译,独立于eIF4G1.1. 这种新的机制调节细胞脱差和转移,影响发育和疾病.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 基因规则 基因规则
背景情况:
- 规范的mRNA翻译依赖于eIF4F复合体,包括eIF4E,eIF4A和eIF4G1.1.
- eIF4G1通过PABPC1结合促进mRNA循环化,并启动核糖体扫描.
- 在乳腺上皮细胞中,CELF1蛋白通过3' UTR结合促进上皮细胞转化为介质细胞转化 (EMT) mRNA的翻译.
研究的目的:
- 研究CELF1在介质细胞转化调节中的作用.
- 阐明CELF1影响mRNA翻译的机制.
- 确定CELF1介导翻译对EMT和转移的影响.
主要方法:
- 通过生物化学分析,研究了CELF1与eIF4E和PABPC1的相互作用.
- 评估了CELF1/eIF4E相互作用中断对mRNA翻译的影响.
- 评估了体外EMT诱导和体内实验性转移.
主要成果:
- 在介质细胞中,CELF1直接与eIF4E和PABPC1结合.
- 这种相互作用促进了含有GRE的mRNAs的eIF4G1独立翻译.
- 破坏CELF1/eIF4E相互作用可以抑制EMT和实验转移.
结论:
- 通过CELF1.1确定了一种新的,非正典的翻译调节模式.
- 这种机制是细胞脱差的基础,并影响转移.
- 表明类似的机制可能调节发育和疾病中的其他过渡性细胞状态.
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