使用树树皮的多3-基酸盐可持续生产:与绿色下游加工集成
João Matias1,2, Thomas Rodrigues1,2, Cristiana A V Torres1,2
1Associate Laboratory i4HB, Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal.
概括
这项研究将树树皮转化为高质量的聚3-基酸生物塑料,使用酶过程. 这种可持续的方法通过将废物提升为有价值的生物聚合物来推进循环生物经济.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 可持续化学 可持续化学
背景情况:
- 细胞生物质是生物聚合物生产的丰富而未充分利用的资源.
- 开发具有成本效益和环保的生物聚合物提取方法对于商业可行性至关重要.
研究的目的:
- 整合林氏纤维素的价值化与生物聚合物提取,用于生产聚3-基酸 (P-3HB).
- 用树树皮作为唯一的细菌培养和P-(3HB) 合成的原料.
- 为了获得高的P-(3HB) 产量和纯度,使用酶性水解和提取.
主要方法:
- 在蒸汽爆炸后,使用细胞分解酶尾酒对树树皮进行水解.
- 在树皮水解剂上种植 *Burkholderia thailandensis* DSM 13276.
- 使用酶提取P-(3HB) 的方法.
- 通过超过回收酶.
主要成果:
- 在细菌细胞中达到7.67g/L的细胞干量和高P-{3HB) 含量 (60.0 wt %).
- 证明了高的聚合物和生长产量 (0.190 gP(3HB)/g糖和0.026 gX/g糖).
- 获得96%的提取效率和100%的生物聚合物纯度,其性能与商业P-(3HB) 相似.
结论:
- 通过使用可持续的酶技术,成功地将白树树皮提升为高质量的P-(3HB) 生物塑料.
- 证明了使用循环生物经济方法用于生物塑料生产的可行性.
- 突出了酵素过程在基纤维素糖化和生物聚合物回收方面的潜力.
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