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Updated: Feb 28, 2026

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

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Published on: March 28, 2014

使用步骤刺激和时间域分析测量表皮细胞单层的次秒外细胞阻抗测量.

Rongming Guo, Athena J Chien, Jake Hawks

bioRxiv : the preprint server for biology

|February 27, 2026

概括

一种新的方法,时间域上皮质阻抗测量 (TEIM),允许次秒测量上皮质屏障功能,克服传统电化学阻抗光谱 (EIS) 的局限性. 这一进步使得细胞模型中快速生物变化的实时监测成为可能.

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科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
电力生理学电力生理学

背景情况:

细胞外电化学阻抗光谱 (EIS) 对于体外表皮研究非常有价值,通过跨细胞电阻 (TER/TEER),跨细胞电容 (TEC) 和膜比 (α) 来评估屏障完整性,形态和极性.
传统的EIS每次测量需要几十秒钟,因为频率范围很广,限制了其捕捉快速生物事件的能力.

研究的目的:

引入时间域上皮质阻抗测量 (TEIM),一种用于上皮细胞单层次次次次秒外细胞阻抗测量的新方法.
为了证明TEIM能够测量关键的上皮细胞参数 (TER,TEC,α),与EIS相比显著改善时间分辨率.

主要方法:

TEIM利用电压瞬态的步骤 (Heaviside函数) 电流激发和时间域分析,消除了对富里埃变换的需求.
测量TER,TEC,α和模型衍生阻抗光谱,采样速度为0.3秒.
使用电路和人类上皮细胞单层 (16HBE,Caco-2) 与EIS验证了TEIM的准确性和精度.

主要成果:

与传统的EIS相比,TEIM在时间解析方面实现了100倍的改进.
与EIS相比,TEIM测量的平均百分比错误很低,在TER的0.17-3.55%,TEC的1.13-8.96%和α的0.59-26.35%之间.
TEIM成功地捕获了用沙波宁治疗的Caco-2细胞中TER和TEC的快速,双指数的短暂变化,这些现象以前无法通过EIS解决.

结论:

TEIM为表皮细胞单层和可能更复杂的体外模型的电生理学研究提供了强大的,高时间分辨率的方法.
这种技术可以在上皮模型中研究快速的生物现象,在疾病建模和治疗开发中推进应用.