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相关概念视频

RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K

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相关实验视频

Updated: Feb 28, 2026

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

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isoespy:一个集成的长读转录组工作流程,用于异型分辨率和可视化.

Ko Ikemoto1, Akihiro Fujimoto1

  • 1Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Bioinformatics advances
|February 27, 2026
PubMed
概括

长读RNA测序揭示了复杂的转录组. 新的isoespy管道整合了异形结构,表达和功能,有助于分析肝细胞癌以了解蛋白质多样性.

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 长读RNA测序 (RNA-seq) 提供了对转录组多样性的深入洞察.
  • 了解单个RNA异构体的功能作用仍然是一个挑战.

研究的目的:

  • 为了引入isoespy,一个新的生物信息学管道.
  • 为长时间读取的RNA-seq数据集成异形结构,微分表达和功能注释.

主要方法:

  • isoespy集成开放的阅读框架预测器.
  • 它将异形表达水平与基因模型相对应.
  • 管道可视化用户提供的位置和非位置特征.

主要成果:

  • 对肝细胞癌数据的应用揭示了异形使用差异.
  • 分析发现预测的蛋白质功能的变化.
  • isoespy促进了对转录组复杂性的解释.

结论:

  • isoespy为转录数据提供了集成的结构和功能可视化.
  • 该管道有助于理解异形水平的功能多样性.

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

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  • 该工具增强了从长时间读取的RNA序列中复杂的转录组的分析.