相关实验视频

Updated: Jun 9, 2026

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Published on: January 30, 2018

在Caco-2细胞中环境聚乙烯微塑料造成的氧化和炎症损伤被多丰富的Limoncella果提取物预防

Stefania Lama¹, Massimo Venditti², Alessandra Biasi²

¹SSD Cryopreservation and B.a.S.C.O., Oncohematology Department AORN Santobono-Pausillipon, Naples, Italy.

Oxidative medicine and cellular longevity

|February 27, 2026

概括

环境聚乙烯微塑料 (PE) 通过增加氧化应激和损害分化,损害肠道细胞. 果聚烯提取物Limoncella (LAPE) 提供了对这种微塑料损伤的保护.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

人类面临环境微塑料 (MP) 的持续暴露.
摄入的MPs可以通过肠道吸收,可能导致不良健康影响.
聚乙烯微塑料 (PE) 是常见的环境污染物.

研究的目的:

研究PE微塑料对分化Caco-2 (D-Caco-2) 肠细胞的有害影响.
评估Limoncella果多提取物 (LAPE) 对PE引起的损伤的保护潜力.

主要方法:

用PE,LAPE或其组合对待D-Caco-2细胞.
评估细胞活力 (MTT试验) 和脂质过氧化 (TBARS试验).
通过免疫光和西部斑点评估F-actin组织,奥克卢丁和NF-κB表达.

主要成果:

暴露PE降低了D-Caco-2细胞活力和受损分化.
PE增加了脂质过氧化和破坏了F-actin组织.
PE改变了关键紧接环蛋白奥克卢丁的表达.

结论:

暴露于PE会诱导氧化应激,导致上皮层-介质细胞过渡和肠道细胞的脱差.
拉佩表现出抗氧化和抗炎性质.
LAPE抵消了PE的有害影响,表明其作为胃肠道保护的营养策略的潜力.

关键词:

果多烯提取物果多烯提取物不同化的Caco-2细胞.环境聚乙烯微塑料环境聚乙烯微塑料表皮细胞-介质细胞过渡.营养保健品营养保健品氧化应激是一种氧化应激.聚醇是一种多醇.紧密的结节紧密的结节

更多相关视频

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

Published on: November 18, 2022

Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model

Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model

Published on: December 2, 2022

相关概念视频

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...