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综合性k-mer和转录基因分析揭示了 Spinacia turkestanica 中假定的性别决定基因
Shoaib Muhammad1, Shuyu Liang1, Tongyu Zhou1
1College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China.
在菜 (Spinacia) 中识别性别决定基因是具有挑战性的. 这项研究使用了一种新的k-mer方法,在菜的性别决定区域 (SDR) 中找到候选的性别决定基因,以帮助分子繁殖.
科学领域:
- 植物遗传学 植物遗传学
- 分子育种是一种分子育种.
- 性别的确定性 性别的确定性
背景情况:
- 解读像菜这样的双胞胎作物的性别决定基因对于分子繁殖至关重要.
- 菜的挑战包括不完整的基因组组合和高的男性和女性基因组相似性,阻碍了基因识别.
研究的目的:
- 在 Spinacia turkestanica 中确定候选的性别决定基因,这是种植的菜的最亲密的亲属.
- 通过采用无参考基因组的k-mer方法来克服以前分析的局限性.
主要方法:
- 使用无基因组基因组的k-mer方法来组装男性特异性读数.
- MAKER管道整合了转录组和蛋白组数据,以预测性别决定区域 (SDR) 中的基因.
- 使用权重基因共表达网络分析和qPCR来识别候选基因.
主要成果:
- 在Spinacia turkestanica.中发现了一个21.5 Mb的SDR.
- 在SDR中预测了226个蛋白质编码基因,包括9个新型基因.
- 鉴定了8个SDR差异表达基因 (DEG),其中3个被确认为候选性别决定因素:TU_SDR00087,TU_SDR000168和TU_SDR000174.
结论:
- 这项研究提供了一个基本的数据集,用于理解在Spinacia的性别决定.
- 已识别的候选基因为未来的菜功能表征和分子育种提供了目标.
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