Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.2K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
The DNA Replication Fork01:02

The DNA Replication Fork

41.6K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.6K
Homologous Recombination02:31

Homologous Recombination

64.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.4K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.4K
Overview
206.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The human limbus functions as an instructive epithelial transition zone.

Research square·2026
Same author

The WEE1 inhibitor azenosertib broadly enhances efficacy of antibody-drug conjugates with topoisomerase I and microtubule inhibitor payloads.

iScience·2026
Same author

Comparative 10-year performance of mammography artificial intelligence, polygenic, and clinical breast cancer risk models in the Kaiser Permanente Research Bank.

Journal of the National Cancer Institute·2026
Same author

National Analysis of Secular Trends and Racial Disparities in Age at Kasai Hepatoportoenterostomy for Infants with Biliary Atresia, 2005-2025.

Annals of surgery·2026
Same author

Material economic hardships are associated with second-year hospitalizations after pediatric liver transplantation: Results from the SOCIAL-Tx study.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2026
Same author

Protocol of a cluster randomized controlled trial of a village health worker care model to reduce cardiovascular disease risk among conflict-affected populations in eastern Myanmar.

Contemporary clinical trials·2026

相关实验视频

Updated: Feb 28, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K

复制蛋白A1对于哺乳动物 oogenesis†期间的DNA损伤修复至关重要.

Xiaosu Miao1, Rui Guo2,3, Andrea Williams1

  • 1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, USA.

Biology of reproduction
|February 27, 2026
PubMed
概括

复制蛋白A (RPA) 对于发育卵细胞中的DNA修复至关重要. 它的缺失会导致DNA损伤,染色体问题和雌性哺乳动物的不孕症.

关键词:
造成的DNA损伤是DNA损伤.染色体对齐是因为染色体对齐.女性生育能力女性生育能力毛囊细胞生成 (Folliculogenesis) 是一种特定于卵子细胞的条件淘汰.

更多相关视频

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

3.3K
Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.5K

相关实验视频

Last Updated: Feb 28, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K
Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

3.3K
Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.5K

科学领域:

  • 生殖生物学 生殖生物学
  • 分子遗传学 分子遗传学
  • 细胞生物学 细胞生物学

背景情况:

  • 卵细胞中未被修复的DNA损伤会导致遗传异常,流产和不孕.
  • 复制蛋白A (RPA) 是一个重要的单链DNA结合复合体,参与DNA过程.

研究的目的:

  • 调查RPA在出生后卵细胞发育过程中的DNA损伤修复中的新型作用.
  • 了解RPA缺乏在卵细胞中的后果.

主要方法:

  • 使用生殖系特异的Cre驱动器 (Ddx4-Cre和Zp3-Cre) 在卵细胞中非激活RPA1 (复制蛋白A1).
  • 评估了RPA复杂分解,DNA损伤,DNA损伤反应激活,染色体对齐和卵泡发育.
  • 分析了参与细胞骨组织的基因的转录水平.

主要成果:

  • RPA1的耗尽导致了RPA复合物的分解和GV阶段卵细胞的严重DNA损伤.
  • 缺少RPA激活了正规的DNA损伤反应通路 (ATM,ATR,DNA-PK,p53).
  • 观察到染色体错位和毛囊生成受损,导致卵细胞数量减少和女性不孕.

结论:

  • 在哺乳动物 oogenesis 期间,RPA 在 DNA 损伤修复中起着至关重要的,以前未知的作用.
  • RPA对于保持卵细胞遗传完整性和女性生育能力至关重要.