Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

929
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
929

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecular-Extrusion-Driven Halogen Homogenization for Efficient Perovskite-Silicon Tandem Solar Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

A multifunctional composite film comprising a citric acid-crosslinked polysaccharide matrix loaded with nutmeg essential oil nanoemulsion: suitable for facilitating active wound healing.

Biomaterials advances·2026
Same author

Potential of Bacillus amyloliquefaciens XSZ-1 as a bio-inoculant for enhancing cotton productivity and health in saline-alkali cotton field soil.

World journal of microbiology & biotechnology·2026
Same author

Anti-fatigue activity of seabuckthorn seed meal derived high Fischer ratio peptides: integrated evidence from murine models and network pharmacology.

International journal of biological macromolecules·2026
Same author

Structural Homology Solid Additives Enhancing Crystallization Thermodynamics for Constructing Ordered Fibrillar Network Toward 20.22% Efficiency Layer-by-Layer Organic Solar Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Stable and Low-Cost Organic Photovoltaics Without Conjugated Donors.

Advanced materials (Deerfield Beach, Fla.)·2026

相关实验视频

Updated: Feb 28, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K

用于活细胞单分子超分辨率成像的1,3-二碳基罗达胺.

Qiang Peng1,2, Yangfan Wang2, Bei Zheng3

  • 1Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang Province, China.

Angewandte Chemie (International ed. in English)
|February 27, 2026
PubMed
概括

研究人员开发了用于超分辨率显微镜的新型1,3-二碳尼尔-罗达胺衍生物. 这些探测器能够精确地对活细胞中的细胞结构和动态进行成像,并且具有高空间和时间分辨率.

关键词:
1,3-二碳烯化合物是什么?罗达胺是一种胺.超高分辨率成像成像技术

更多相关视频

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K

相关实验视频

Last Updated: Feb 28, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K

科学领域:

  • 化学生物学 化学生物学
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 1,3-二碳化合物具有多功能化学特性,可应用于各种科学领域.
  • 罗达胺光体在生物成像中被广泛使用.
  • 单分子定位显微镜 (SMLM) 需要先进的探针来进行高分辨率的活细胞成像.

研究的目的:

  • 为了合成和描述新型的1,3-二碳尼尔-罗达胺衍生物.
  • 评估这些衍生品对于单分子局部化显微镜 (SMLM) 的适用性.
  • 为了证明这些探头用于活细胞超分辨率成像和动态跟踪的应用.

主要方法:

  • 1,3-二乙烯基支架与罗达胺光体的整合.
  • 利用热力学和光化学特性进行稀疏定位和受控的光氧化.
  • 使用点击化学,HaloTag标签和脂向特定标签.
  • 超高分辨率成像和活细胞中细胞组件的动态跟踪.

主要成果:

  • 成功构建了具有可调节性质的1,3-二碳尼尔-罗达胺衍生物.
  • 展示了稀疏的局部化,可调节的发射器密度和自动触发的光氧化级联.
  • 实现了超高分辨率成像和动态跟踪actin,内 плазма网膜,线粒体和血膜.
  • 解析了filopodia动态,在20分钟的时间内具有22nm的空间精度和20秒的时间分辨率.

结论:

  • 开发的1,3-二碳尼尔-罗达胺衍生物是SMLM的有效探针.
  • 这些探测器有助于在活细胞中的细胞结构的高保真性,长期动态跟踪.
  • 这项研究展示了一种用于推进超分辨率显微镜技术的新方法.