相关实验视频

Updated: Feb 28, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

作为光学探针的结合性橄电解质

Samuel J W Chan^1,2, Ji-Yu Zhu^1,2, Guillermo C Bazan^1,2

¹Institute for Digital Molecular Analytics and Science, Nanyang Technological University, Singapore 636921, Singapore.

Accounts of chemical research

|February 27, 2026

概括

结合性橄电解质 (COEs) 是一种新型的光学探针,可可视化和跟踪细胞膜的特性. 这些探测器为生物成像和疾病检测提供了新的途径.

科学领域:

* 有机化学有机化学
* 生物物理生物物理
* 材料科学材料科学

背景情况:

* 光学探头对于研究生物和化学系统至关重要.
* 结合的电解质 (COEs) 是合成的两性质,具有 π-结合的骨干和带电组.
*COEs可以模仿脂质双层,促进它们融入细胞膜.

研究的目的:

* 用光学探针来描述COE的设计策略.
* 突出其在阐明和跟踪细胞膜特性中的应用.
* 探索它们在生物成像和疾病诊断方面的潜力.

主要方法:

* 设计和合成可调节电子和光物理性质的COE.
* COEs的应用用于检测和可视化各种尺度 (细胞,囊泡,有机体) 的脂质膜.
*利用先进的显微镜技术 (例如,双光子,STED,FLIM) 进行高分辨率成像和动态过程分析.

主要成果:

*COEs有效地可视化了各种生物系统中的脂质膜,从单细胞到活体动物模型.
* COEs作为高分辨率时空成像的非线性光学探针.
*使用COE的光终身成像显微镜 (FLIM) 揭示了张力,刚性和组成等动态膜性质.

结论:

*COEs代表了开发基础研究和生物成像的先进光学探针的多功能平台.
* 量身定制的COE可以充当功能探测器,可预测地响应其当地的环境.
*进一步开发COEs有望为疾病检测和诊断创造新的工具.

更多相关视频

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

UV–Vis Spectroscopy of Conjugated Systems

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in...