相关实验视频
Updated: Aug 13, 2026

09:37
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
Published on: October 26, 2009
37.7K
关于细胞外囊泡交叉连接的超分子水凝的配方和表征的协议
Artemis Margaronis1, James S Wang1, Yara A Aguilar1
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
STAR protocols
|February 27, 2026
概括
这项研究介绍了一种创建可注射水凝的协议,使用细胞外囊泡 (EV) 作为交叉连接器. 这些生物活性水凝为各种生物医学应用提供可调整的机制.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 组织工程是组织工程.
背景情况:
- 细胞外囊泡 (EVs) 正在成为生物过程中的关键组成部分.
- 由于其多功能性质,水凝被广泛用于生物医学应用.
- 控制水凝机制对于模仿本地组织环境至关重要.
研究的目的:
- 详细介绍一个由细胞外囊泡 (EVs) 交联的可注射水凝的配方协议.
- 通过聚合物-EV设计来调整水凝的机械性能.
- 为了指导这些新型水凝的风学特征和分析.
主要方法:
- 制备基修饰的基基甲基纤维素 (HPMC) 聚合物.
- 将HPMC聚合物与EV混合在一起,形成超分子凝网络.
- 风湿学性状鉴定,以评估水凝的特性和机制.
主要成果:
- 通过使用EV作为超分子交叉连接器成功制备可注射水凝.
- 通过调整聚合物-电动汽车设计参数来演示可调节的水凝机制.
- 确定质性质和设计参数之间的相关性.
结论:
- EV交叉连接的水凝为先进的生物医学应用提供了一个有希望的平台.
- 该协议为设计具有定制机械性能的生物活性水凝提供了一个框架.
- 调整水凝机制的能力有助于在各种再生医学策略中使用它们.
更多相关视频
12:07Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
Published on: April 16, 2018
14.1K
09:39Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
Published on: February 7, 2021
9.0K
相关概念视频
Membrane Fluidity
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Gap Junctions
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
Membrane Fluidity
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Gap Junctions
The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...