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通过液体染色学 (LC-MS/MS):单实验室验证,第一项行动:2025.07

Toshiya Matsubara¹, Yusuke Mizuno¹, Eishi Imoto¹

¹Shimadzu Scientific Instruments, Inc, 7102 Riverwood Dr., Columbia, MD, 21046 USA.

Journal of AOAC International

|February 27, 2026

概括

一种新的液体染色学双重质谱法 (LC-MS/MS) 方法准确量化了多种食品中的30种聚和 perfluoroalkyl 物质 (PFAS). 这种经过验证的方法符合食品安全分析的性能要求.

科学领域:

环境化学环境化学
分析化学分析化学
食品安全食品安全

背景情况:

聚和 perfluoroalkyl 物质 (PFAS) 是持久的,生物累积的化有机化学物质.
PFAS污染可以通过来自受影响环境的植物和动物在食品供应中发生.
关键的PFAS化合物包括 perfluorooctanoic acid (PFOA) 和 perfluorooctanesulfonic acid (PFOS) 这两种化合物.

研究的目的:

执行一个LC-MS/MS方法的单个实验室验证 (SLV),用于在各种食品矩阵中确定30种PFAS.
为了比较该方法的性能与AOAC国际标准方法性能要求 (SMPR®) 2023.003.
建立一种可靠的分析方法,用于在广泛的食品中检测PFAS.

主要方法:

食品样本使用乙和QuEChERS试剂提取.
通过固体相提取 (SPE) 实现净化,然后在下缩 (依赖于矩阵).
使用同位素稀释与LC-MS/MS进行量化,评估回收,精度和线性.

主要成果:

在LC-MS/MS方法中,大多数分析对象的线性反应在0.01-25μg/kg之间.
方法验证包括恢复,精度,保留时间,空白,离子比率和信号对噪声的评估.
染色分离有效地解决了酸的潜在干扰.

结论:

开发的LC-MS/MS方法成功地达到所需的量化极限 (LOQ),回收和精度标准.
该方法使用一组单一的操作条件,以便在实验室之间有效的可转移性.
简化提取和最小的溶剂/劳动力要求使其成为一个具有成本效益的分析解决方案.

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