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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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相关实验视频

Updated: Mar 1, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

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代谢性RNA标记-启用时间解析单细胞RNA测序.

Kun Yin1,2, Shichao Lin1, Chaoyong Yang1,2

  • 1MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Fujian Key Laboratory of Chemical Biology, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Accounts of chemical research
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PubMed
概括
此摘要是机器生成的。

用单细胞RNA测序 (scRNA-seq) 进行代谢RNA标记,可以发现动态基因表达. 像Well-TEMP-seq和scDUAL-seq这样的新方法可以改善细胞和动物中的RNA动态分析.

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Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • 细胞基因表达是异质和动态的,对于理解健康和疾病至关重要.
  • 单细胞RNA测序 (scRNA-seq) 提供了快照,但缺乏RNA动态的时间分辨率.
  • 了解RNA合成,降解和细胞命运需要时间解析的基因表达数据.

研究的目的:

  • 审查代谢RNA标签的进展,基于时间解析的scRNA-seq.
  • 突出在单细胞水平上分析RNA动态的方法.
  • 讨论RNA动态研究中化学工具的未来方向.

主要方法:

  • 与scRNA-seq平台集成代谢性RNA标签 (例如4-thioridine) 的整合.
  • 开发Well-TEMP-seq以提高吞吐量,效率和成本效益.
  • 实施scDUAL-seq用于同时监测RNA合成和降解.
  • 在动物模型中应用体内代谢RNA标记 (Dyna-vivo-seq).
  • 时间解析的scRNA-seq与空间转录组的整合,用于空间时间分析.

主要成果:

  • 代谢性RNA标记能够对数千个基因的RNA动态进行无偏见的捕获.
  • 好的TEMP-seq提高了性能,并降低了时间解析的scRNA-seq. 的成本.
  • scDUAL-seq准确地测量RNA动力学,并揭示RNA合成和降解之间的相互作用.
  • Dyna-vivo-seq将单细胞RNA动态研究扩展到生物体.
  • 时空融合为组织微环境中的基因调节提供了洞察力.

结论:

  • 基于代谢RNA标记的scRNA-seq是剖析细胞异质性和动态的一个强大的工具.
  • 最近的技术进步为RNA调节机制提供了前所未有的洞察力.
  • 化学工具的未来发展对于推进单细胞RNA动态分析和生物医学应用至关重要.