在禁食和运动后,对PERM1的调节和在人类骨肌肉中选择基因
Eveline S Menezes1, Hashim Islam2, Benjamin B Arhen3
1Queen's University, School of Kinesiology and Health Studies, Kingston, Ontario, Canada; 19esm2@queensu.ca.
概括
禁食并没有改变骨肌肉中的PERM1通路. 然而,急性和慢性高强度运动增加了与肌肉新陈代谢和线粒体功能相关的关键蛋白质和基因,这表明运动会影响PERM1信号.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 骨肌肉生物学 骨肌肉生物学
背景情况:
- 过氧体增殖器激活受体马协活性剂1-α (PGC-1α) 是线粒体生物发生的主调节器.
- PERM1 (也称为NR4A3) 在调节肌肉能量代谢,收缩功能和线粒体生物生成方面发挥着至关重要的作用.
研究的目的:
- 研究禁食和急性高强度运动 (HIIE) 对骨肌肉信号通路的影响.
- 检查慢性高强度间歇训练 (HIIT) 对PERM1及其目标基因的影响.
- 评估PERM1在人类骨肌肉中的细胞局部.
主要方法:
- 来自现有研究的肌肉活检样本的二次分析.
- 使用RT-PCR和西式斑点测试来量化mRNA和蛋白质水平.
- 采用免疫标记,显微镜和亚细胞分离来确定PERM1定位.
主要成果:
- 禁食并没有显著改变与PERM1相关的途径.
- HIIE增加了p-p38MAPK的蛋白质水平和PERM1和PGC-1α的mRNA水平.
- 六周的HIIT提高了PERM1异型2和CKMT2蛋白水平,PERM1在周核区域和线粒体中被发现.
结论:
- 运动,特别是HIIT,会影响人类骨肌肉中与PERM1相关的通路.
- 只有PERM1通路的某些组成部分在研究条件下似乎被保留.
- 需要进一步的研究,以充分阐明PERM1在人类骨肌肉生理学中的功能作用.
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