通过ComQXPA的定数感应系统调节Bacillus subtilis的抗李斯特菌活性
Katarina Šimunović1, Hana Sobočan1, Tjaša Danevčič1
1Department of Microbiology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, Ljubljana 1000, Slovenia.
Microbiological research
|February 27, 2026
概括
细菌细菌PS-216有效地打击Listeria monocytogenes,一个危险的食物传播病原体. 这种益生菌.
科学领域:
- 食品微生物学 食品微生物学
- 细菌遗传学 细菌遗传学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是严重的食物传播疾病 (李斯特菌病) 的重要原因之一.
- 这种病原体形成了弹性生物膜,增加了食品环境中的污染风险.
- 细菌细菌PS-216表现出抗菌性质,特别是对抗阴性细菌.
研究的目的:
- 评估Bacillus subtilisPS-216对Listeria物种的疗效. 为了评估Bacillus subtilisPS-216对Listeria物种的疗效.
- 调查定数感应 (QS) 和其受管制的抗微生物在B. subtilisPS-216的抗李斯特菌活性中的作用.
- 为了评估B. subtilis的抗菌膜潜力PS-216.6.
主要方法:
- 使用野生型B. subtilis PS-216和对Listeria monocytogenes和Listeria innocua. QS缺乏突变物进行了抗菌和抗菌膜测定.
- 对QS组件 (comQ,srfAA,bacA) 的基因表达分析是使用单种和共同种的YFP促进体融合进行的.
- 无垢菌被用作L. monocytogenes的替代模型.
主要成果:
- B. subtilis PS-216在共同培养中对Listeria物种显示出显著的日志减少 (5.9-6.7日志).
- 该ComQXPA QS系统对于抗Listeria活性至关重要,它调节了表面素和细菌素的产生.
- B. subtilis PS-216有效地抑制了Listeria生物膜的形成,表面素在防止草类型生物膜方面发挥了关键作用.
结论:
- 细菌细菌PS-216具有强大的抗微生物和抗菌膜活动对利斯特菌物种.
- 对于控制李斯特菌来说,ComQXPA的定数感应系统及其产品 (表面素,细菌素) 是必不可少的.
- B. subtilis PS-216显示出作为一种广谱益生菌的承诺,用于管理食品环境中的L. monocytogenes等食物传播病原体.
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