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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Updated: Jul 6, 2026

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

用AFM和超分辨率成像来估计多隔间细胞弹性的最佳设计.

Emilio A Mendiola¹, Brandon K Walther², Anahita Mojiri³

¹Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA.

Computer methods and programs in biomedicine

|February 27, 2026

概括

此摘要是机器生成的。

这项研究引入了一个新的计算框架,使用原子力显微镜 (AFM) 准确测量细胞组件的机械性质,如细胞核和细胞质. 这提高了机械生物学研究的一致性.

关键词:

原子力显微镜的原子力显微镜.细胞弹性细胞弹性细胞粘弹性细胞粘弹性核膜是一种核膜.实验的最佳设计实验的最佳设计.

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AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Published on: July 24, 2014

Live Cell Imaging during Mechanical Stretch

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

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Last Updated: Jul 6, 2026

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

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Published on: July 24, 2014

Live Cell Imaging during Mechanical Stretch

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科学领域:

细胞生物力学细胞生物力学
机械生物学机械生物学
生物物理学的生物物理.

背景情况:

估计亚细胞区的机械性质对于理解健康和疾病中的细胞行为至关重要.
现有的方法,如原子力显微镜 (AFM) 面临的挑战,在准确性和可重现性,由于测量变化和错误的反向问题.
量化核膜,细胞质和核质的机械特性是特别复杂的.

研究的目的:

开发一个集成的实验计算框架,以实现最佳的反向方法设计.
通过提高准确性和可重复性来估计多间隔细胞的机械性能.
尽量减少财产估计对AFM探测位置的依赖.

主要方法:

使用超分辨率成像构建了人类静脉内皮细胞 (HUVEC) 的3D计算模型.
采用反向建模方法,将实验数据与超弹性构成模型相匹配,并结合大变形非线性.
利用粘性-超弹性模拟来研究粘性效应.

主要成果:

成功量化了核质,核膜和细胞质的机械性质.
实现了这些属性估计对负载条件的最小依赖.
展示了一种方法,可以提高机械性质估计的可重复性.

结论:

开发的框架有助于标准化亚细胞结构的生物力学表征.
提高机械生物学的研究的一致性和可重复性.
有助于更好地了解机械传导在疾病进展中的作用.