有机酸 - 一种双重作用模式,影响宿主和病原体的完整性,以减少沙门氏菌感染
Diana Marcu1, Igori Balta1, Ozan Gundogdu2
1Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, 300645 Timisoara, Romania.
Microbial pathogenesis
|February 27, 2026
概括
有机酸 (As) 的混合物通过抑制细菌入侵,生长和炎症,有效地预防了的沙门氏菌 Typhimurium 感染. 这种化合物还可以减少细胞损伤,并恢复肠道屏障功能.
科学领域:
- 兽医微生物学 兽医微生物学
- 禽类的健康 禽类的健康
- 食品安全 食品安全
背景情况:
- 沙门氏菌Typhimurium对家禽健康和食品安全构成重大威胁.
- 了解感染预防机制对于制定有效的控制策略至关重要.
研究的目的:
- 阐明有机酸 (AuraShield - As) 在初级白内障上皮细胞 (PECC) 和白内障活检中预防沙门氏菌Typhimurium感染的机制.
- 在体外和体外评估亚的亚抑制度的疗效.
主要方法:
- 确定的最小抑制度 (MIC) 和最小杀菌度 (MBC) 对于S. Typhimurium.
- 评估了亚抑制性As度对细菌附着,入侵,移动性和生物膜形成的影响.
- 测量了LDH释放,炎症性细胞因子水平 (IFN,IL-1β,IL-6,IL-8),细胞内Ca2+水平和体电阻 (TEER).
主要成果:
- 据证明,MIC为0.50%,MBC为1%对S. Typhimurium.
- 亚抑制度 (0.25%) 显著降低了PECC细胞和白内障活检中的细菌附着,入侵,移动性和生物膜形成.
- 通过减少LDH释放和降低关键炎症媒介的调节来减弱细胞毒性和炎症.
- 用As治疗降低了细胞内Ca2+水平,并在感染细胞和活检中恢复了TEER.
结论:
- 在家禽模型中,AuraShield (As) 有效地抑制了沙门氏菌 Typhimurium 感染.
- 该机制涉及破坏细菌毒性因子,降低细胞毒性,调节宿主炎症反应.
- 似乎可以调节细胞内水平,有助于其对沙门氏菌感染的保护作用.
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