Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Local Anesthetics: Differential Sensitivity of Nerve Fibers01:24

Local Anesthetics: Differential Sensitivity of Nerve Fibers

1.5K
Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...
1.5K
Local Anesthetics: Common Agents and Their Applications01:23

Local Anesthetics: Common Agents and Their Applications

1.1K
Local anesthetics (LAs) are commonly used for various applications in medical and dental procedures. Some of the common agents used are cocaine, lidocaine, and bupivacaine.
Cocaine is an ester of benzoic acid and methylecgogine. It is used to anesthetize and vasoconstrict locally. Currently, it is used primarily for topical applications. It is beneficial for surgeries on the upper respiratory tract, providing anesthesia and shrinking the mucosa. Cocaine in the form of cocaine hydrochloride is...
1.1K
Local Anesthetics: Clinical Application as Intravenous Regional Anesthesia01:16

Local Anesthetics: Clinical Application as Intravenous Regional Anesthesia

1.6K
Intravenous regional anesthesia or the Bier block technique is used to anesthetize a specific limb or extremity. It uses exsanguinated or blood-drained vessels to transport local anesthetics or LAs to the peripheral nerve trunks. Lidocaine without vasoconstrictors like epinephrine is most commonly used for this technique. Other drugs used are prilocaine, ropivacaine, and chloroprocaine. Bupivacaine is not recommended for this technique due to its high cardiac toxicity.
One of the advantages of...
1.6K
Local Anesthetics: Clinical Application as Surface, Infiltration, and Conduction Block Anesthesia01:30

Local Anesthetics: Clinical Application as Surface, Infiltration, and Conduction Block Anesthesia

2.3K
Depending on the target organ, local anesthetics (LAs) can be administered via various routes. In surface anesthesia, LAs are applied directly to the surface of the skin or mucous membranes. It is widely used for topical skin numbing before venipuncture or minor surgical procedures. Commonly used surface local anesthetics are lidocaine or benzocaine sprays or creams. Surface anesthesia occurs within 5 minutes and lasts for about 60 minutes. One of the main disadvantages of topical anesthesia is...
2.3K
Local Anesthetics: Clinical Application as Spinal Anesthesia01:11

Local Anesthetics: Clinical Application as Spinal Anesthesia

1.6K
Spinal anesthetics are given during lower abdomen and limb surgeries to block sensory and motor neurons. They are administered in the mid to low lumbar regions, primarily acting on the cauda equina's nerve roots. The blockade level depends on the local anesthetic (LA) concentration. Usually, low LA concentrations are sufficient to block sensory fibers, while only high LA concentrations block motor fibers. Other factors like injection volume and speed, the patient's posture, and the drug...
1.6K
Local Anesthetics: Chemistry and Structure-Activity Relationship01:30

Local Anesthetics: Chemistry and Structure-Activity Relationship

6.9K
Local anesthetics (LAs) are drugs that induce a temporary loss of sensation in a limited body area, preventing pain. Cocaine was the first local anesthetic discovered in the late 19th century. Cocaine is a benzoic acid ester obtained from the leaves of coca shrubs and was often used for its psychotropic effects. Cocaine was first isolated in 1860 by Albert Niemann. Sigmund Freud studied the physiological actions of cocaine. Carl Koller later introduced it into clinical practice in 1884 as a...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Correction: Do we still need a canary in the coal mine for laboratory animal facilities? A systematic review of environmental health monitoring versus soiled bedding sentinels.

PloS one·2026
Same author

Analysis of IVC Microenvironments During an Extended Cage-Change Interval in Rats (Rattus norvegicus).

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same author

Aquatic Advancements: Refining and Validating a Zebrafish Survival Blood Collection Technique.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same author

Multiple Longitudinal Tracts in the Cephalopod Arm Sensorimotor System.

The Journal of comparative neurology·2026
Same author

Enhancing Rodent Welfare, Cost Savings, and Efficiency: Implementation and Review of Data-Driven Improvements.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same author

Feasibility and preliminary results of modeling a clinically meaningful anastomotic leak in pigs.

International journal of surgery (London, England)·2026

相关实验视频

Updated: Mar 1, 2026

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior
12:20

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

Published on: June 10, 2013

22.0K

在章鱼 bimaculoides 评估局部麻醉,以提供止痛.

Joanna N Copio1, Natalie G Schulz2, Clifton W Ragsdale2

  • 11Animal Resources Center and Department of Surgery, The University of Chicago, Chicago, Illinois.

Journal of the American Association for Laboratory Animal Science : JAALAS
|February 27, 2026
PubMed
概括

对于章鱼来说,有效的疼痛管理至关重要,但尚未研究. 利多卡因和布皮瓦卡因注射在章鱼手臂再生中没有提供止痛,这表明在头足动物研究中需要新的止痛方法.

关键词:
ANC,轴神经带的神经带鱼,人造海水,人造海水R2,右手臂的第二个右臂.fASW,过的人造海水.

更多相关视频

Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay
07:39

Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay

Published on: July 26, 2022

4.3K
Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management
09:03

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management

Published on: March 28, 2025

1.2K

相关实验视频

Last Updated: Mar 1, 2026

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior
12:20

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

Published on: June 10, 2013

22.0K
Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay
07:39

Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay

Published on: July 26, 2022

4.3K
Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management
09:03

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management

Published on: March 28, 2025

1.2K

科学领域:

  • 兽医麻醉学 兽医麻醉学
  • 头足动物研究 头足动物研究
  • 动物福利 动物福利

背景情况:

  • 头足动物研究正在扩大,但对这些无脊椎动物缺乏有效的疼痛管理和止痛药.
  • 目前美国没有联邦法规针对头足动物的疼痛缓解.
  • 章鱼 bimaculoides 在研究中越来越多地被使用,这需要在疼痛管理方面考虑道德问题.

研究的目的:

  • 评估利多卡因,布皮瓦卡因和延长释放布皮瓦卡因在Octopus bimaculoides中缓解疼痛的可行性和有效性.
  • 评估局部麻醉剂的潜在不良影响,实用性和组织病理学结果.
  • 研究这些麻醉剂对疼痛感知,健康指标和手臂再生的影响.

主要方法:

  • 一项涉及青少年章鱼 (Octopus bimaculoides) 进行手臂再生的两期研究.
  • 第一阶段:对药物管理效应的试点评估 (n=49).
  • 第二阶段:使用行为指标 (姿势,步态,全身),健康监测和伤口愈合评估 (n=34) 进行有效性测试.

主要成果:

  • 所有动物都在手术后立即出现过渡性,统计学上显著的手臂姿势和步态变化,在72小时内消失.
  • 在治疗组之间没有观察到健康指标 (体重变化,呼吸率) 或伤口愈合/手臂再生的显著差异.
  • 局部麻醉剂 (利多卡因,布皮瓦卡因) 没有证明在为章鱼手臂再生提供止痛效果.

结论:

  • 目前的局部麻醉方案在章鱼研究模型中对控制疼痛无效.
  • 这项研究突出了头足动物镇痛方面的关键差距,强调需要开发替代疼痛管理策略.
  • 对新型止痛疗法的进一步研究对于头足动物研究的伦理进步至关重要.