相关实验视频

Updated: Mar 1, 2026

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

Published on: May 6, 2014

oxPAPC介导的 lncRNA CYP1B1-AS1 来自树突细胞加速动脉样硬化

Yuheng Cheng^1,2, Lang Ni¹, Changhao Ke²

¹Zunyi Medical University, Zunyi, China.

Journal of cellular and molecular medicine

|February 27, 2026

概括

氧化1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (oxPAPC) 诱导树突细胞 (DC) 衍生的长非编码RNA (lncRNAs),加速动脉样硬化 (AS). 这项研究确定了CYP1B1-AS1作为一个通过NFATC2.2.的积极调节循环促进AS的关键lncRNA.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
心血管研究研究心血管研究

背景情况:

动脉样硬化 (AS) 涉及氧化脂质,树突细胞 (DC) 和长非编码RNA (lncRNAs).
在AS病变发生过程中,oxPAPC诱导的DC衍生的lncRNAs的具体作用尚不清楚.

研究的目的:

调查oxPAPC诱导的DC衍生的lncRNAs对AS的贡献.
阐明这一过程背后的监管机制.

主要方法:

用oxPAPC刺激DCs,然后使用RNA测序进行转录基因分析.
在ChIP-Seq和RNA下拉试验中,确定了lncRNA CYP1B1-AS1和NFATC2.2.之间的相互作用.
用小鼠模型 (Apoe-/-小鼠) 来评估AS进展.

主要成果:

CYP1B1-AS1被确定为DCs中的一个关键的oxPAPC诱导的lncRNA,其小鼠同类Gm33055增强了Cyp1b1的表达.
oxPAPC促进了NFATC2的核转位,它与CYP1B1-AS1促进体结合;然后CYP1B1-AS1与NFATC2相互作用,形成一个积极的反循环.
采用表达m-CYP1B1-AS1的DCs的转移加速了Apoe-/-小鼠中的AS进展.

结论:

一个DC衍生 lncRNA介导的调节轴涉及CYP1B1-AS1和NFATC2促进动脉样硬化.
这个轴代表了AS治疗的潜在治疗目标.

关键词:

在CYP1B1-AS中.这是一个LncRNARNA.动脉样硬化动脉样硬化树突细胞的树突细胞.oxPAPCPCPCPC的使用方法

相关概念视频

Atherosclerosis I: Introduction

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...

Inflammation

Inflammation

Coronary Artery Disease I: Introduction

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...

Peripheral Artery Disease I: Introduction

Peripheral Artery Disease I: Introduction

Peripheral artery disease (PAD) predominantly results from atherosclerosis, which involves the accumulation of fatty deposits, or plaques, within the walls of arteries. This causes them to narrow and harden, significantly reducing blood flow. PAD predominantly affects the legs, particularly the arteries supplying the thighs and calves. In rare cases, it may involve other arteries, including those in the arms.Etiology of PAD:The principal cause of PAD is atherosclerosis, which results from fatty...