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分类学距离和scriptaid对猪类动物iSCNT胚胎发育的影响

Hai-Jun Liu^1,2, Ka Wai Wong³, Xiaoxiao Ma⁴

¹Endangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore, 138673, Singapore.

Scientific reports

|February 27, 2026

概括

跨物种体细胞核转移 (iSCNT) 的效率受到分类学距离的限制. 脚本治疗通过调节线粒体基因表达来改善猪iSCNT胚胎发育,可能克服"分类学墙".

科学领域:

生殖生物学生殖生物学
遗传学是一个遗传学.
保护生物学保护生物学

背景情况:

跨物种体细胞核转移 (iSCNT) 对危物种的保护具有前景,但效率较低.
在iSCNT的发育失败往往与捐赠物种和接受物种之间的分类学距离的增加有关.

研究的目的:

调查分类学距离对猪SCNT胚胎发育的影响.
评估Scriptaid治疗对改善iSCNT结果的疗效.
阐明分子机制,特别是线粒体基因调节,是iSCNT成功或失败的基础.

主要方法:

使用来自不同分类学距离 (物种内,物种间,子家族间) 的供体细胞进行了iSCNT.
用Scriptaid处理的胚胎和评估的胚胎细胞率和发育时间.
进行了转录组分析,以确定差异表达基因 (DEG) 和丰富通路 (GO,KEGG).

主要成果:

囊胚细胞率随着分类学距离的增加而显著下降 (27.8%至0%).
斯克里普泰德治疗改善了胚芽细胞的发育率,加速了细胞分裂,并提高了猪iSCNT胚胎中的H3K9ac水平.
转录组分析揭示了线粒体功能和氧化酸化通路的显著丰富,NDUF和ND基因家族的失调与分类学距离和Scriptaid治疗相关.

结论:

分类学距离对猪类动物的iSCNT胚胎发育有负面影响,主要是通过核编码 (NDUF) 和线粒体编码 (ND) 基因的失调.
脚本治疗通过调节线粒体基因表达来部分挽救iSCNT发育缺陷,提供一种克服"分类学墙"的潜在策略.
线粒体功能和氧化酸化是iSCNT在不同分类学距离上的成功的关键决定因素.

关键词:

线粒体基因基因是线粒体基因.脚本是用来写的.猪类动物猪类动物分类学距离的距离.转录组的概况分析在iSCNT胚胎发育过程中.

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